Diet-induced obesity and pancreatic islet blood flow in the rat: a preferential increase in islet blood perfusion persists after withdrawal of the diet and normalization of body weight.
Diet-induced obesity and pancreatic islet blood flow in the rat: a preferential increase in islet blood perfusion persists after withdrawal of the diet and normalization of body weight.
复制标题
饮食引起的肥胖和大鼠胰岛血流量：在停止饮食和体重正常化后，胰岛血灌注持续增加。
作者：
A. M. Svensson;C. Hellerström;L. Jansson
本研究的目的是评价饮食诱导肥胖对正常Wistar大鼠胰岛血流灌注的影响。此外，我们还调查了在体重恢复到正常饮食后，任何与肥胖相关的胰岛血流变化在多大程度上可以逆转。年轻的成年雌性Wistar大鼠除了标准的颗粒食物外，还被提供了可口的混合高热量饮食(自助餐厅饮食)。年龄匹配的对照组大鼠只接受标准颗粒饲料。4周后，饮食处理组大鼠的体重比对照组增加了约15%。所有饮食处理的大鼠都降低了糖耐量，升高了血清胰岛素浓度，但麻醉饮食处理的大鼠和对照大鼠的基础血糖浓度相似。用微球技术测量全胰腺和胰岛血流量。自助式饮食的大鼠胰岛血流量和胰岛血流量分数均增加(P<；0.01)，而全胰腺血流灌注量与对照组相近。在第二个实验中，大鼠接受了4周的自助餐厅饮食，然后只喂标准颗粒食物3周，而对照组接受7周的标准饮食。在此期间，总体重、腹膜后脂肪垫重量和糖耐量与对照组相似。全胰腺血流量与对照组相比无明显变化。但胰岛血流量(P<；0.01)和血流量分数(P<；0.01)均增加。我们的结论是，饮食诱导的肥胖大鼠与糖耐量下降、高胰岛素血症以及胰岛绝对和部分血液灌注量的特异性增加有关。尽管体重和糖耐量正常化，这种增加在停止饮食后至少持续3周。
The aim of the present study was to evaluate the effects of diet-induced obesity on pancreatic islet blood perfusion in normal Wistar rats. Furthermore, we investigated to what extent any obesity-associated changes in islet blood flow could be reversed after reversion to a normal diet with normalization of body weight. Young adult female Wistar rats were offered a palatable mixed high-caloric diet (cafeteria diet) in addition to standard pelleted chow. Age-matched control rats received standard pelleted chow only. After 4 weeks the diet-treated rats had a body weight of approximately 15% more than that of the controls. All diet-treated rats had decreased glucose tolerance and increased serum insulin concentrations, but basal blood glucose concentrations were similar in anesthetized diet-treated and control rats. Whole pancreatic and islet blood flow rates were measured with a microsphere technique. The islet blood flow as well as fractional islet blood flow were increased (P < 0.01) in rats fed the cafeteria diet, while blood perfusion of the whole pancreas was similar to that of the control rats. In a second experiment, rats received the cafeteria diet for 4 weeks and were then fed standard pelleted food alone for another 3 weeks, while controls received standard diet for 7 weeks. After this period total body weight, retroperitoneal fat pad weight and glucose tolerance were similar to those of the controls. Whole pancreatic blood flow was unchanged as compared with that of control rats. However, both islet blood flow (P < 0.01) and fractional blood flow (P < 0.01) were increased. We conclude that diet-induced obesity in rats is associated with decreased glucose tolerance, hyperinsulinemia and a specific increase in absolute and fractional islet blood perfusion. This increase persists for at least 3 weeks after the diet is withdrawn despite normalization of body weight and glucose tolerance.
自助餐厅饮食——不适合研究生热作用的工具。
DOI：
10.1093/jn/117.2.227
发表时间：
1987
期刊：
The Journal of nutrition
影响因子：
--
作者：
Moore,BJ
通讯作者：
Moore,BJ