Genomic DNA amplification from a single bacterium
Genomic DNA amplification from a single bacterium
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DOI：
10.1128/aem.71.6.3342-3347.2005
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发表时间：
2005-06-01
影响因子：
4.4
通讯作者：
Lasken, RS
中科院分区：
文献类型：
--
作者：
Raghunathan, A;Ferguson, HR;Lasken, RS
使用phi 29 DNA聚合酶，通过多重置换扩增（MDA）反应，从单个流式分选的细菌细胞中扩增基因组DNA约50亿倍。从扩增的DNA中可以准确地测定16S rRNA基因的662 bp片段。MDA方法为研究不可培养的微生物提供了新的策略。
Genomic DNA was amplified about 5 billion-fold from single, flow-sorted bacterial cells by the multiple displacement amplification (MDA) reaction, using phi 29 DNA polymerase. A 662-bp segment of the 16S rRNA gene could be accurately sequenced from the amplified DNA. MDA methods enable new strategies for studying nonculturable microorganisms.