Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR.
Clinical application of quantitative analysis for detection of hematogenous spread of hepatocellular carcinoma by real-time PCR.
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实时荧光定量PCR检测肝细胞癌血行扩散定量分析的临床应用
DOI：
10.3892/ijo.18.3.527
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发表时间：
2001
影响因子：
5.2
通讯作者：
M. Monden
中科院分区：
文献类型：
--
作者：
A. Miyamoto;H. Nagano;M. Sakon;Y. Fujiwara;Y. Sugita;H. Eguchi;M. Kondo;I. Arai;O. Morimoto;K. Dono;K. Umeshita;S. Nakamori;M. Monden
为了检测肝细胞癌患者肿瘤细胞的血行播散，采用了逆转录-聚合酶链式反应方法。在本研究中，我们采用实时定量聚合酶链式反应(Real-time PCR)技术对甲胎蛋白(AFP)信使RNA进行了定量。对23例肝细胞癌切除患者、31例健康志愿者、10例肝硬变患者和5例肝切除患者外周血中AFP信使RNA进行了定量检测。在实时荧光聚合酶链式反应中，在任何对照组中都检测不到荧光。相反，在23例肝细胞癌患者的39份血液标本中，有10份检测到荧光信号。AFP阳性状态与多发性肝内结节的存在显著相关。在甲胎蛋白阳性的8例患者中，有3例出现肝内外复发。在8例AFP阳性患者中，这3例患者的AFP信使RNA含量较高。用LightCycler建立的实时荧光定量聚合酶链式反应方法可检测到AFP信使RNA，提示该方法可用于检测肝癌患者血液中的少量肿瘤细胞。
In order to detect a hematogenous spread of tumor cells in patients with hepatocellular carcinoma, reverse transcription-polymerase chain reaction assay has been used. In this study, we quantified alpha-fetoprotein (AFP) messenger RNA by real-time PCR approach using LightCyclertrade mark technique. AFP messenger RNA in the blood from 23 hepatocellular carcinoma patients undergoing hepatic resection, 31 healthy volunteers, 10 patients with liver cirrhosis and 5 patients underwent hepatectomy except for hepatocellular carcinoma was quantitated. In the real-time PCR, fluorescence was undetectable in any of the controls. On the contrary, fluorescent signals were detected in 10 out of 39 blood specimens collected from 23 HCC patients. AFP-positive status was significantly associated with the existence of multiple intrahepatic nodules. Out of 8 cases with AFP-positive status, intra- and/or extra-hepatic recurrence has been observed in 3 cases. The quantities of AFP messenger RNA in these 3 cases were relatively high among 8 cases with AFP-positive status. AFP messenger RNA was detectable by newly developed real-time PCR approach with LightCycler and it is suggested that this approach could be applicable in detection of small amounts of tumor cells in the blood of HCC patients.