Expression of Proliferating Cell Nuclear Antigen (PCNA) in Oral Submucous Fibrosis: An Immunohistochemical Study
Expression of Proliferating Cell Nuclear Antigen (PCNA) in Oral Submucous Fibrosis: An Immunohistochemical Study
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DOI：
10.7860/jcdr/2015/13046.5885
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发表时间：
2015-05-01
影响因子：
0.2
通讯作者：
Narayanappa, Umashree
中科院分区：
文献类型：
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作者：
Keshav, Roopavathi;Narayanappa, Umashree
背景与目的：增殖细胞核抗原（PCNA）的表达与细胞分裂密切相关。PCNA在基底层上的表达被认为是口腔粘膜异型增生的标志，表明在这些病变中存在一种特殊的增殖细胞状态。口腔粘膜下纤维性变（OSMF）被认为是一种癌前病变，有可能转化为口腔鳞状细胞癌（OSCC）。材料与方法：收集经病理证实的40例口腔鳞状细胞癌组织，经福尔马林固定、石蜡包埋，观察PCNA在口腔鳞状细胞癌中的表达情况，并与正常口腔鳞状细胞癌进行比较。研究组又分为两组：30例OSMF和10例OSCC。这些样本收集自班加罗尔政府牙科学院和研究所口腔颌面病理学系。结果：40例乳腺癌组织中PCNA均呈阳性表达。OSMF和OSCC中阳性细胞均以基底层和基底上层较多，而OSCC中阳性细胞以浅层较多。所有OSMF均在基底层和基底层上呈阳性表达，仅77%的OSMF在浅表层呈不同程度的PCNA阳性表达，23%的OSMF在浅表层无阳性表达。结论：OSMF增殖层中PCNA阳性细胞比例增加。这种生物标志物提供了对疾病的生物学行为的深入了解，证实了其潜在的恶性性质，也可能在化疗试验中提供中间终点。
Background and objectives: Expression of Proliferating Cell Nuclear Antigen (PCNA) well-correlates with the cell division. The suprabasal expression of PCNA is considered to be a marker of dysplastic oral mucosa indicating a special proliferative cellular state in those lesions. Oral submucous fibrosis (OSMF) is considered to be a premalignant condition with potential to get transformed into oral squamous cell carcinoma (OSCC). The present study was done to assess the PCNA expression in different grades and in different layers of epithelium of OSMF and to compare the expression of PCNA in OSMF with OSCC.Materials and Methods: A group of 40 histopathologically diagnosed, formalin fixed, paraffin embedded tissue samples were included in study. The study group was further divided into 2 groups; 30 OSMF and 10 OSCC. Theses samples were collected from Department of Oral and Maxillofacial Pathology, Government Dental College and Research Institute, Bangalore. The samples were subjected to immunohistochemical method using indirect immunoenzyme LSAB method.Results: All 40 cases showed positivity for PCNA. The percentage of positive cells was more in basal and suprabasal layer both in OSMF and OSCC, whereas the greater percentage of positive cells was seen in the superficial layer of OSCC. All OSMF cases showed positive expression in basal and suprabasal layer and only 77% of cases showed positive PCNA expression in the superficial layer with variable immunoreactivity and 23% did not show any positive staining in the superficial layer. The percentage of positive cells and the intensity of staining increased as the disease severity increased in OSMF.Conclusion: Increased proportion of cells expressed PCNA staining which are in proliferative layers of OSMF. This biomarker provides an insight into the biological behaviour of the condition that substantiates its potentially malignant nature and may also provide an intermediate end point in chemotherapeutic trials.