Intramural Purkinje Cell Network of Sheep Ventricles as the Terminal Pathway of Conduction System
Intramural Purkinje Cell Network of Sheep Ventricles as the Terminal Pathway of Conduction System
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DOI：
10.1002/ar.20827
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
James, Thomas N.
中科院分区：
文献类型：
--
作者：
Ryu, Shonosuke;Yamamoto, Shoji;James, Thomas N.
为了确定心脏电信号传导的解剖学基础，特别是寻找心室内传导途径的壁内末端，利用浦肯野细胞的特征形态作为组织学标记，将绵羊心脏与人类心脏进行了比较。在15只羊和5例非心源性死亡的尸体解剖中，组织学检查了房室结、其远端传导通路、室间隔和左右心室游离壁的浦肯野或浦肯野型细胞的患病率。在从室间隔和心室游离壁获得的跨壁切片（沿心脏基底至心尖轴）中检查心肌组织劈裂。在选定的绵羊中，通过几乎整个隔膜的连续组织学切片被用作传导途径的三维重建的基础，特别是内部浦肯野细胞网络。在绵羊心室壁心肌中发现浦肯野细胞，而在人心室壁心肌中未发现浦肯野细胞。在羊隔中，每个壁内浦肯野细胞组成一个三维网络，贯穿壁心肌，其近端与束分支的心内膜下延伸连接，远端与普通工作的肌细胞偶尔形成连接。浦肯野细胞网络可能作为终末传导途径参与心室兴奋。浦肯野细胞之间的单个连接包含穿壁定向的连接，这将有利于信号传导通过绵羊心脏的分裂跨越结构障碍。病变心脏的心肌结构改变可破坏包括跨壁定向在内的神经网络连接。中国农业科学，29(2):12-22,2009。(c) 2008 Wiley-Liss, Inc。
To identify the anatomical basis for cardiac electrical signal conduction, particularly seeking the intramural terminals of conduction pathway within the ventricles, sheep hearts were examined compared with human hearts utilizing the characteristic morphology of Purkinje cells as a histological marker. In 15 sheep and five human autopsies of noncardiac death, prevalence of Purkinje or Purkinje-type cells were histologically examined in the atrioventricular node, its distal conduction pathway, the interventricular septum, and the right- and left-ventricular free walls. Myocardial tissue cleavages were examined in the transmural sections (along cardiac base-to-apex axis) obtained from the septum and ventricular free walls. Serial histological sections through virtually the entirety of the septum in selected sheep were used as the basis of a three-dimensional reconstruction of the conduction pathway, particularly of the intramural Purkinje cell network. Purkinje cells were found within the mural myocardium of sheep ventricles whereas no intramural Purkinje-type cell was detected within the human ventricles. In the sheep septum, every intramural Purkinje cell composed a three-dimensional network throughout the mural myocardium, which proximally connected to the subendocardial extension of the bundle branches and distally formed an occasional junction with ordinary working myocytes. The Purkinje-cell network may participate in the ventricular excitation as the terminal conduction pathway. Individual connections among the Purkinje cells contain the links of through-wall orientation which would benefit the signal conduction crossing the architectural barriers by cleavages in sheep hearts. The myocardial architectural changes found in diseased hearts could disrupt the network links including those with transmural orientation. Anat Rec, 292:12-22, 2009. (c) 2008 Wiley-Liss, Inc.