INHIBITION OF ROUS-SARCOMA VIRUS-REPLICATION AND CELL TRANSFORMATION BY A SPECIFIC OLIGODEOXYNUCLEOTIDE
INHIBITION OF ROUS-SARCOMA VIRUS-REPLICATION AND CELL TRANSFORMATION BY A SPECIFIC OLIGODEOXYNUCLEOTIDE
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DOI：
10.1073/pnas.75.1.280
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发表时间：
1978-01-01
影响因子：
11.1
通讯作者：
STEPHENSON, ML
中科院分区：
文献类型：
--
作者：
ZAMECNIK, PC;STEPHENSON, ML
将三聚体d（a - a - t - g - g - t - a - a - a - t - g - g）添加到感染劳斯肉瘤病毒的鸡胚成纤维组织培养物中，该三聚体d与劳斯肉瘤病毒35S RNA 3′和5′重复末端序列的13个核苷酸互补。病毒产生受到抑制。显然，tridecamer及其3‘’和5'‘-羟基末端被阻断的对应体进入鸡成纤维细胞，与35S RNA 3‘’和5’末端的末端重复序列杂交，并干扰病毒产生和细胞转化的一个或多个步骤。可能的作用位点是原病毒DNA中间体的循环化步骤和翻译的起始，后者将在未来的交流中描述。
The tridecamer d(A-A-T-G-G-T-A-A-A-A-T-G-G), which is complementary to 13 nucleotides of the 3''- and 5''-reiterated terminal sequences of Rous sarcoma virus 35S RNA, was added to chick embryo fibroblast tissue cultures infected with Rous sarcoma virus. Inhibition of virus production resulted. Apparently, the tridecamer and its counterpart with blocked 3''- and 5''-hydroxyl termini enter the chick fibroblast cells, hybridize with the terminal reiterated sequences at the 3'' and 5'' ends of the 35S RNA, and interfere with 1 or more steps involved in viral production and cell transformation. Likely sites of action are the circularization step of the proviral DNA intermediate, and the initiation of translation, the latter to be described in a future communication.