Macrophage response to cross-linked and conventional UHMWPE
Macrophage response to cross-linked and conventional UHMWPE
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DOI：
10.1016/s0142-9612(03)00056-5
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发表时间：
2003-07-01
期刊：
影响因子：
14
通讯作者：
Shanbhag, AS
中科院分区：
文献类型：
--
作者：
Sethi, RK;Neavyn, MJ;Shanbhag, AS
为了防止磨损碎屑引起的骨质溶解和无菌性松动，已经开发出具有改善的耐磨性的交联超高分子量聚乙烯（UHMWPE）。髋关节模拟器研究表明，这些新材料的磨损率非常低，导致其广泛的临床应用。然而，该材料的生物相容性尚不清楚。我们研究了巨噬细胞对交联UHMWPE（XLPE）的反应，并将其与传统UHMWPE（CPE）以及其他临床使用的骨科材料（如钛合金（TiAlV）和钴铬合金（CoCr））进行了比较。将人外周血单核细胞和鼠巨噬细胞作为介导植入物周围炎症的细胞的替代物，培养在由试验材料制成的定制设计的唇状圆盘上，以分离细胞。在24和48小时收集培养物上清液，并分析细胞因子如IL-1 α、IL-1 β、TNF-α和IL-6。从贴壁细胞中提取总RNA，并使用定性RT-PCR分析基因表达。在这两种体外模型中，在交联聚乙烯和常规聚乙烯上培养的巨噬细胞释放类似水平的细胞因子，其也类似于对照组织培养皿上的水平。在TiAlV和CoCr合金上培养的巨噬细胞释放显著更高水平的细胞因子。来自所有供体的人单核细胞在相同表面上培养时释放的细胞因子的大小不同。个体供体对TiAlV和CoCr表面的反应的可变性可能反映了个体对类似刺激的不同反应，并可能揭示了对特定材料的易感性。(C)2003爱思唯尔科技有限公司版权所有。
To prevent wear debris-induced osteolysis and aseptic loosening, cross-linked ultra-high molecular weight polyethylene's (UHMWPE) with improved wear resistance have been developed. Hip simulator studies have demonstrated very low wear rates with these new materials leading to their widespread clinical use. However, the biocompatibility of this material is not known. We studied the macrophage response to cross-linked UHMWPE (XLPE) and compared it to conventional UHMWPE (CPE) as well as other clinically used orthopaedic materials such as titanium-alloy (TiAlV) and cobalt-chrome alloy (CoCr). Human peripheral blood monocytes and murine macrophages, as surrogates for cells mediating peri-implant inflammation, were cultured onto custom designed lipped disks fabricated from the test materials to isolate cells. Culture supernatants were collected at 24 and 48 h and analyzed for cytokines such as IL-1alpha, IL-1beta, TNF-alpha and IL-6. Total RNA was extracted from adherent cells and gene expression was analyzed using qualitative RT-PCR. In both in vitro models, macrophages cultured on cross-linked and conventional polyethylene released similar levels of cytokines, which were also similar to levels on control tissue culture dishes. Macrophages cultured on TiAlV and CoCr-alloy released significantly higher levels of cytokines. Human monocytes from all donors varied in the magnitude of cytokines released when cultured on identical surfaces. The variability in individual donor responses to TiAlV and CoCr surfaces may reflect how individuals respond differently to similar stimuli and perhaps reveal a predisposed sensitivity to particular materials. (C) 2003 Elsevier Science Ltd. All rights reserved.