Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi
Effect of inoculum size on single-cell oil production from glucose and xylose using oleaginous yeast Lipomyces starkeyi
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DOI：
10.1016/j.jbiosc.2017.12.020
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发表时间：
2018-06-01
影响因子：
2.8
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
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作者：
Juanssilfero, Ario B.;Kahar, Prihardi;Kondo, Akihiko
产油微生物可以将底物如二氧化碳、糖和有机酸转化为单细胞油（SCO）。在产油微生物中，斯达氏油脂酵母是一种特别适合的宿主，因为它具有令人印象深刻的天然能力，包括利用各种碳源的能力。在这项工作中，潜在的L。通过使用葡萄糖和/或木糖作为碳源改变接种量来研究starkeyi NBRC 10381在合成氮限制性矿物培养基（-NMM）中产生SC 0。使用葡萄糖和木糖作为混合碳源的发酵产生最高的生物质产量为40.8 g/L，并实现了84.9%（w/w）的脂质含量。当分别使用葡萄糖或木糖时，达到的脂质含量的总和分别为79.6%（w/w）和85.1%（w/w）。然而，葡萄糖的生物质产量高于木糖（分别为303对28.7g/L）。本研究描述了在不同接种量下，使用葡萄糖和/或木糖作为碳源，首次同时实现更高水平的细胞质量和脂质生产。(C)2017年，日本生物技术学会。All rights reserved.
Oleaginous microbes can convert substrates such as carbon dioxide, sugars, and organic acids to single-cell oils (SCOs). Among the oleaginous microorganisms, Lipomyces starkeyi is a particularly well-suited host given its impressive native abilities, including the capability to utilize a wide variety of carbon sources. In this work, the potential of L. starkeyi NBRC10381 to produce SCOs in a synthetically nitrogen-limited mineral medium (-NMM) was investigated by differing the inoculum size using glucose and/or xylose as a carbon source. Fermentation using glucose and xylose as mixed carbon sources generated the highest production of biomass at 40.8 g/L, and achieved a lipid content of 84.9% (w/w). When either glucose or xylose was used separately, the totals for achieved lipid content were 79.6% (w/w) and 85.1% (w/w), respectively. However, biomass production was higher for glucose than for xylose (303 vs. 28.7 g/L, respectively). This study describes the first simultaneous achievement of higher levels of cell mass and lipid production using glucose and/or xylose as the carbon sources in different inoculum sizes. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.