Suppression of delayed-type hypersensitivity and IgE antibody responses to ovalbumin by intranasal administration of Escherichia coli heat-labile enterotoxin B subunit-conjugated ovalbumin.
Suppression of delayed-type hypersensitivity and IgE antibody responses to ovalbumin by intranasal administration of Escherichia coli heat-labile enterotoxin B subunit-conjugated ovalbumin.
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DOI：
10.1016/s0264-410x(96)00135-1
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发表时间：
1997-02
期刊：
影响因子：
5.5
通讯作者：
S. Tamura;E. Hatori;T. Tsuruhara;C. Aizawa;T. Kurata
中科院分区：
文献类型：
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作者：
S. Tamura;E. Hatori;T. Tsuruhara;C. Aizawa;T. Kurata
已知口服少量抗原结合的霍乱毒素B亚单位可诱导对抗原的耐受性。在本实验中，是否过敏原结合大肠杆菌热不稳定毒素B亚单位（LTB）鼻腔给药诱导的耐受性在BALB/c小鼠进行了检查。单次给药少量的LTB偶联的卵清蛋白（OVA）抑制诱导迟发型超敏反应和IgE抗体反应的OVA，这是在鼻内给药LTB偶联的OVA后，胃肠外给药。通过向LTB偶联的OVA中加入全毒素来消除抗原特异性抑制。通过鼻腔给药少量与粘膜结合分子缀合的过敏原诱导的抑制可能适用于预防过敏的发展。
Oral administration of a small amount of antigen conjugated cholera toxin B subunit is known to induce tolerance to the antigen. In the present experiments, whether nasal administration of allergen conjugated to Escherichia coli heat-labile toxin B subunit (LTB) induced tolerance was examined in BALB/c mice. A single administration of a small amount of LTB-coupled ovalbumin (OVA) suppressed the induction of delayed-type hypersensitivity and IgE antibody responses to OVA which was administered parenterally after nasal administration of LTB-coupled OVA. The antigen-specific suppression was abrogated by the addition of the holotoxin to LTB-coupled OVA. The suppression, induced by nasal administration with a small amount of allergen conjugated to a mucosa-binding molecule, may be applicable for preventing the development of allergy.