Comprehensive Dissection of Metabolic Changes in Albino and Green Tea Cultivars
Comprehensive Dissection of Metabolic Changes in Albino and Green Tea Cultivars
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白化茶和绿茶品种代谢变化的综合剖析
DOI：
10.1021/acs.jafc.7b05623
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发表时间：
2018-02-28
影响因子：
6.1
通讯作者：
Chen, Liang
中科院分区：
文献类型：
--
作者：
Li, Chun-Fang;Ma, Jian-Qang;Chen, Liang
白化茶品种是茶树的特殊突变体，叶色为白色或黄色。本研究采用气相色谱质谱法和超高效液相色谱质谱法对“安吉白茶”、“黄金芽”和“白鸡冠”三个白化茶品种以及龙井43和土顶大白茶两个绿茶品种进行代谢物分析。多变量分析显示，白化品种和绿色品种叶片中的代谢物表型存在显着差异。差异代谢相关途径包括半乳糖代谢、色氨酸代谢、苯丙素生物合成和类黄酮生物合成。对于白化品种的幼叶，糖（山梨醇和赤藓糖）和氨基酸（主要是脯氨酸、异亮氨酸、鸟氨酸、天冬氨酸、苏氨酸和缬氨酸）浓度增加，而没食子儿茶素和表没食子儿茶素没食子酸酯浓度下降。这些结果揭示了不同叶色的茶树品种之间代谢谱的差异。随着叶片的发育，白化品种老叶中黄酮类化合物的浓度大幅增加。
Albino tea cultivars are special mutants of tea plants with white or yellow leaf color. In this study, three albino tea cultivars, including 'Anji Baicha', 'Huangjinya', and 'Baijiguan', and two green tea cultivars, longjing 43' and Tuding Dabaicha', were applied to metabolite profiling by gas chromatography mass spectrometry and ultraperformance liquid chromatography mass spectrometry. Multivariate analyses revealed significantly different metabolite phenotypes in leaves among albino cultivars and green cultivars. The differential metabolite-related pathways included galactose metabolism, tryptophan metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis. For the young leaves of albino cultivars, the sugar (sorbitol and erythrose) and amino acid (mainly proline, isoleucine, ornithine, aspartic acid, threonine, and valine) concentrations increased, whereas gallocatechin and epigallocatechin gallate concentrations decreased. These results reveal the divergence in metabolic profiling between tea plant cultivars with different leaf colors. With the development of leaves, the concentrations of flavonoids increased largely in the older leaves of albino cultivars.