Effect of the Jianpi Bushen Prescription on expressions of the Wnt3a and Cyclin D1 genes in radiation-damaged mice.
Effect of the Jianpi Bushen Prescription on expressions of the Wnt3a and Cyclin D1 genes in radiation-damaged mice.
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DOI：
10.4238/2013.october.3.1
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发表时间：
2013-10
期刊：
影响因子：
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通讯作者：
D. He;J. Xiao;Y. Zhang;H. Lin;X. Ding;Y. Tu
中科院分区：
文献类型：
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作者：
D. He;J. Xiao;Y. Zhang;H. Lin;X. Ding;Y. Tu
观察健脾补肾方对辐射损伤小鼠Wnt 3a和Cyclin D1基因表达的影响。昆明种小鼠经单次全身照射9天后，建立辐射损伤模型。将小鼠分为辐射组、低剂量（100%）JBP组、高剂量（200%）JBP组和鲨肝醇组（阳性对照），每天给药2次，共9天。实时荧光定量PCR和Western blot检测骨髓单个核细胞Wnt 3a mRNA和蛋白表达，原位杂交检测Cyclin D1 mRNA表达。辐射损伤模型组Wnt3a表达较其他各组明显下调（P <0.05）。放射损伤模型组Cyclin D1 mRNA表达阳性细胞率和粒-巨噬细胞集落数较其他各组均显著降低（P <0.05）。JBP低剂量组Wnt3a mRNA和蛋白表达、骨髓细胞Cyclin D1 mRNA表达阳性细胞率、粒-巨噬细胞集落数均显著高于JBP高剂量组（P <0.05）。综上所述，JBP通过激活Wnt3a信号通路，促进Cyclin D1的转录和表达，对辐射诱导的骨髓细胞起到保护作用。
The effects of the traditional Chinese drug Jianpi Bushen Prescription (JBP) were investigated on expressions of Wnt3a and Cyclin D1 genes in radiation-damaged mice. The radiation damage model was induced in Kumming mice by single total body irradiation treatment for 9 days. Mice were divided into the radiation group, low-dose (100%) JBP group, high-dose (200%) JBP group, or batyl alcohol group (positive control), which were administered twice a day for 9 days. mRNA and protein expressions of Wnt3a were detected in bone marrow mononuclear cells by real-time polymerase chain reaction and Western blot, whereas Cyclin D1 mRNA was detected by in situ hybridization. Wnt3a expressions were significantly downregulated in the radiation damage model group compared with all other groups (P < 0.05). The positive cell rate of Cyclin D1 mRNA expression and the number of granulocyte macrophage colonies were significantly decreased in the radiation damage model group relative to all other groups (P < 0.05). Furthermore, mRNA and protein expressions of Wnt3a, the positive cell rate of Cyclin D1 mRNA expression in bone marrow cells, and the number of granulocyte macrophage colonies were all significantly higher in the low-dose JBP group than in the high-dose JBP group (P < 0.05). In summary, JBP plays a protective role on radiation-induced bone marrow through the activation of the Wnt3a signaling pathway, and promotes the transcription and expression of Cyclin D1.