FULLY H-2 INCOMPATIBLE CHIMERA, T-CELLS OF DONOR ORIGIN CAN RESPOND TO MINOR HISTOCOMPATIBILITY ANTIGENS IN ASSOCIATION WITH EITHER DONOR OR HOST H-2 TYPE
FULLY H-2 INCOMPATIBLE CHIMERA, T-CELLS OF DONOR ORIGIN CAN RESPOND TO MINOR HISTOCOMPATIBILITY ANTIGENS IN ASSOCIATION WITH EITHER DONOR OR HOST H-2 TYPE
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DOI：
10.1084/jem.148.1.84
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发表时间：
1978-01-01
影响因子：
15.3
通讯作者：
MIRKWOOD, G
中科院分区：
文献类型：
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作者：
MATZINGER, P;MIRKWOOD, G
用BALB基因小鼠制备了完全H-2不相容的辐射嵌合体。这些嵌合小鼠在体内免疫C57BL/10Sn （B10）背景的组织相容性抗原，这些抗原与亲本H-2单倍型中的任何一种相关。他们的脾细胞随后在体外用相同的次要抗原增强。对少量抗原检测到强烈的h -2限制性细胞毒活性。限制的特异性可能是针对供体或宿主的H-2单倍型，这取决于用于启动或增强的细胞。在这些小鼠中也发现了交叉启动。完全同种异体放射嵌合体可以产生H-2限制性T[胸腺源]细胞对次要组织相容性(H)抗原的反应。这些结果与最近获得的针对病毒抗原的对比结果进行了讨论。
Fully H-2 incompatible radiation chimeras were prepared using BALB congenic mice. Such chimeric mice were immunized in vivo against histocompatibility antigens of the C57BL/10Sn (B10) background in association with either of the parental H-2 haplotypes. Their spleen cells were subsequently boosted in vitro with the same minor antigens. Strong H-2-restricted cytotoxic activity against minor antigens was detected. The specificity of the restriction could be to the H-2 haplotype of the donor or the host depending on the cells used for priming or boosting. Cross priming was also demonstrated in these mice. Fully allogenic radiation chimeras can produce H-2-restricted T[thymus-derived]-cell responses to minor histocompatibility (H) antigens. The results were discussed in relation to contrasting results recently obtained against viral antigens.