Rapid and Sensitive Detection of H1N1/2009 Virus from Aerosol Samples with a Microfluidic Immunosensor
Rapid and Sensitive Detection of H1N1/2009 Virus from Aerosol Samples with a Microfluidic Immunosensor
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DOI：
10.1177/2211068213504205
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发表时间：
2014-06-01
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影响因子：
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通讯作者：
Yoon, Jeong-Yeol
中科院分区：
文献类型：
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作者：
Kwon, Hyuck-Jin;Fronczek, Christopher F.;Yoon, Jeong-Yeol
甲型H1N1/2009流感是一种高传染性、迅速传播的空气传播疾病，需要进行近乎实时的监测，最好是以微流控形式进行监测。然而，由于H1N1在气溶胶样本中的浓度极低，加上尘埃颗粒的干扰，这样的演示很难找到。在这项工作中，我们测量了带有光波导通道的微流体装置的Mie散射强度，其中抗体偶联的乳胶珠与目标H1N1抗原进行免疫凝集。通过仔细优化光学参数，我们能够最大限度地增加乳胶免疫凝集的Mie散射，同时最小化灰尘颗粒的背景散射。气溶胶样本是从1：10的模拟教室中使用按钮空气采样器采集的，在那里，雾化器产生气溶胶，模拟人类咳嗽。用光谱仪和手机摄像头作为光学探测器，对实际气溶胶样品的检出限分别为1和10pg/m L。这些方法比其他方法灵敏几个数量级。微流控免疫传感器读数与逆转录聚合酶链式反应结果一致。S进样时间为30分钟，微流控检测时间为5分钟。
Influenza A H1N1/2009 is a highly infectious, rapidly spreading airborne disease that needs to be monitored in near real time, preferably in a microfluidic format. However, such demonstration is difficult to find as H1N1 concentration in aerosol samples is extremely low, with interference from dust particles. In this work, we measured Mie scatter intensities from a microfluidic device with optical waveguide channels, where the antibody-conjugated latex beads immunoagglutinated with the target H1N1 antigens. Through careful optimizations of optical parameters, we were able to maximize the Mie scatter increase from the latex immunoagglutinations while minimizing the background scatter from the dust particles. The aerosol samples were collected from a 1:10 mock classroom using a button air sampler, where a nebulizer generated aerosols, simulating human coughing. The detection limits with real aerosol samples were 1 and 10 pg/mL, using a spectrometer or a cell phone camera as an optical detector, respectively. These are several orders of magnitudes more sensitive than the other methods. The microfluidic immunosensor readings are in concordance with the results of reverse transcription polymerase chain reaction. The assay time was 30 s for sampling and 5 min for the microfluidic assay.