The mitotic history and radiosensitivity of developing oligodendrocytes in vitro.
The mitotic history and radiosensitivity of developing oligodendrocytes in vitro.
复制标题
体外发育少突胶质细胞的有丝分裂历史和放射敏感性。
DOI：
10.1016/0012-1606(84)90096-4
复制
发表时间：
1984
影响因子：
2.7
通讯作者：
Silberberg,DH
中科院分区：
文献类型：
--
作者：
Hirayama,M;Eccleston,PA;Silberberg,DH
通过脉冲追踪暴露大鼠胎儿脊髓或脑的游离细胞于[3H]胸苷（TdR）和未标记的TdR，研究表明不表达半乳糖脑苷（GalC）的少突胶质前体先分裂，后分化表达GalC。更成熟的GalC+少突胶质细胞的增殖率明显低于它们的CalC -前体细胞。研究发现，少突胶质前体细胞对[3H]TdR辐照极为敏感。暴露于0.03 μCi/ml 24小时被证明是有害的，特别是在出生前的关键时期。这一关键期与少突胶质前体细胞分裂的高峰期相对应。
By use of pulse-chase exposure of dissociated cells of rat fetal spinal cord or brain to [3H]thymidine (TdR) and unlabeled TdR it has been shown that oligodendroglial precursors which do not express galactocerebroside (GalC) divide first and later differentiate to express GalC. The rate of proliferation of more mature GalC+oligodendrocytes is considerably lower than that of their CalC−precusors. It has been found that oligodendrocyte precursor cells are extremely sensitive to [3H]TdR irradiation. Exposure to as little as 0.03 μCi/ml for 24 hr proved to be harmful, particularly during a critical period before birth. This critical period corresponded to the peak of division of oligodendrocyte precursor cells.