Using ABCG2-molecule-expressing side population cells to identify cancer stem-like cells in a human ovarian cell line
Using ABCG2-molecule-expressing side population cells to identify cancer stem-like cells in a human ovarian cell line
复制标题
DOI：
10.1042/cbi20100347
复制
发表时间：
2011-03-01
影响因子：
3.9
通讯作者：
Gu, Ning
中科院分区：
文献类型：
--
作者：
Dou, Jun;Jiang, Cuilian;Gu, Ning
CSC（癌症干细胞）是肿瘤内的一小部分细胞，具有干细胞的特征，被认为负责对放化疗产生耐药性。通过干细胞特征鉴定 CSC 可能具有相关的临床意义。在本研究中，通过 FACS 从人卵巢癌细胞系中分选 SP（侧群）细胞，以确定是否存在癌症干细胞样 SP 细胞。在 A2780 细胞中检测到极小部分 SP 细胞 (2.6%)。 SP细胞具有高增殖活性、在软琼脂和培养基中具有显着的自我更新能力、ABCG2高表达、体外对长春碱耐药、Balb/c裸鼠致瘤能力强等特点。结论：A2780细胞系中存在少量ABCG2高表达的SP细胞。这些细胞具有癌症干细胞样细胞的特征，此类人类SP细胞的鉴定和克隆有助于改善卵巢癌患者的治疗方法。
CSCs (cancer stem cells) are a small subset of cells within a tumour that possesses the characteristics of stem cells and are considered to be responsible for resistance to chemoradiation. Identification of CSCs through stem cell characteristics might have relevant clinical implications. In this study, SP (side population) cells were sorted from a human ovarian cancer cell line by FACS to determine whether cancer stem cell-like SP cells were present. A very small fraction of SP cells (2.6%) was detected in A2780 cells. SP cells possessed the following characteristics: highly proliferative activity, marked ability for self-renewal in soft agar and culture medium, high expression of ABCG2, drug resistance to vinblastine in vitro, and strong tumourigenic potential in Balb/c nude mice. It is concluded that there exists in the A2780 cell line a small number of SP cells with high expression of ABCG2. The cells have the characteristics of cancer stem-like cells, and identification and cloning of such human SP cells can help in improving therapeutic approaches to ovarian cancer in patients.