The Microbial Detection Array Combined with Random Phi29-Amplification Used as a Diagnostic Tool for Virus Detection in Clinical Samples
The Microbial Detection Array Combined with Random Phi29-Amplification Used as a Diagnostic Tool for Virus Detection in Clinical Samples
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DOI：
10.1371/journal.pone.0022631
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发表时间：
2011-08-10
期刊：
影响因子：
3.7
通讯作者：
Fomsgaard, Anders
中科院分区：
文献类型：
--
作者：
Erlandsson, Lena;Rosenstierne, Maiken W.;Fomsgaard, Anders
用于临床样本中敏感和特异性诊断病毒检测的常用技术是PCR，其可以在一次测定中鉴定一种或多种病毒。然而，含有所有人类病原体探针的诊断微阵列可以取代数百个单独的PCR反应，并消除对可疑病原体的明确临床假设的需要。我们已经建立了这样一个诊断平台，用于随机扩增和随后的微阵列鉴定临床样品中的病毒病原体。我们表明，Phi29聚合酶扩增的一组不同的临床样本产生足够的病毒材料成功识别的微生物检测阵列，展示了广谱病原体检测的微阵列技术的潜力。我们的结论是，这种方法检测DNA和RNA病毒，存在于同一样品中，以及区分不同的病毒亚型。我们提出这种检测用于临床样品中病毒的诊断分析。
A common technique used for sensitive and specific diagnostic virus detection in clinical samples is PCR that can identify one or several viruses in one assay. However, a diagnostic microarray containing probes for all human pathogens could replace hundreds of individual PCR-reactions and remove the need for a clear clinical hypothesis regarding a suspected pathogen. We have established such a diagnostic platform for random amplification and subsequent microarray identification of viral pathogens in clinical samples. We show that Phi29 polymerase-amplification of a diverse set of clinical samples generates enough viral material for successful identification by the Microbial Detection Array, demonstrating the potential of the microarray technique for broad-spectrum pathogen detection. We conclude that this method detects both DNA and RNA virus, present in the same sample, as well as differentiates between different virus subtypes. We propose this assay for diagnostic analysis of viruses in clinical samples.