Fabrication of hybrid nanocomposite scaffolds by incorporating ligand-free hydroxyapatite nanoparticles into biodegradable polymer scaffolds and release studies.
Fabrication of hybrid nanocomposite scaffolds by incorporating ligand-free hydroxyapatite nanoparticles into biodegradable polymer scaffolds and release studies.
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DOI：
10.3762/bjnano.6.227
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发表时间：
2015
影响因子：
3.1
通讯作者：
Beke S
中科院分区：
文献类型：
--
作者：
Farkas B;Rodio M;Romano I;Diaspro A;Intartaglia R;Beke S
本文报道了一种结合液体中脉冲激光烧蚀和掩模投影准分子激光立体光刻（MPExSL）制备羟基磷灰石（HA）和可生物降解聚合物的自支撑复合薄膜的光学制造方法。无配体HA纳米颗粒制备超快激光烧蚀的HA目标在溶剂中，然后将纳米颗粒分散到液体聚合物树脂的光固化过程之前，使用MPExSL。该树脂是聚（富马酸丙二醇酯）（PPF），一种可光聚合、可生物降解的材料。将聚合物与富马酸二乙酯以7：3 w/w共混以调节树脂粘度。通过SEM和纳米压痕，分别进行制造的混合薄膜的结构和机械性能的评价，而化学和降解研究进行通过热重分析，和FTIR。发现光固化效率依赖于纳米颗粒浓度。MPExSL工艺产生具有嵌入的HA纳米颗粒的稳定且均匀分散的PPF薄膜。在这里，不可能通过改变激光参数来调整支架的刚度和硬度，尽管对于常规PPF支架观察到了这一点。最后，逐渐释放的羟基磷灰石纳米粒子薄膜生物降解的报告。
We report on the optical fabrication approach of preparing free-standing composite thin films of hydroxyapatite (HA) and biodegradable polymers by combining pulsed laser ablation in liquid and mask-projection excimer laser stereolithography (MPExSL). Ligand-free HA nanoparticles were prepared by ultrafast laser ablation of a HA target in a solvent, and then the nanoparticles were dispersed into the liquid polymer resin prior to the photocuring process using MPExSL. The resin is poly(propylene fumarate) (PPF), a photo-polymerizable, biodegradable material. The polymer is blended with diethyl fumarate in 7:3 w/w to adjust the resin viscosity. The evaluation of the structural and mechanical properties of the fabricated hybrid thin film was performed by means of SEM and nanoindentation, respectively, while the chemical and degradation studies were conducted through thermogravimetric analysis, and FTIR. The photocuring efficiency was found to be dependent on the nanoparticle concentration. The MPExSL process yielded PPF thin films with a stable and homogenous dispersion of the embedded HA nanoparticles. Here, it was not possible to tune the stiffness and hardness of the scaffolds by varying the laser parameters, although this was observed for regular PPF scaffolds. Finally, the gradual release of the hydroxyapatite nanoparticles over thin film biodegradation is reported.