Hydrogen peroxide produced by NADPH oxidases increases proline accumulation during salt or mannitol stress in Arabidopsis thaliana
Hydrogen peroxide produced by NADPH oxidases increases proline accumulation during salt or mannitol stress in Arabidopsis thaliana
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DOI：
10.1111/nph.13550
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发表时间：
2015-12-01
期刊：
影响因子：
9.4
通讯作者：
Savoure, Arnould
中科院分区：
文献类型：
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作者：
Ben Rejeb, Kilani;Lefebvre-De Vos, Delphine;Savoure, Arnould
许多植物积累脯氨酸，一个兼容的渗透调节剂，以响应各种环境胁迫，如水分亏缺和盐度。在某些胁迫反应中，植物产生过氧化氢（H2 O2），介导许多生理和生化过程。为了研究胁迫诱导的脯氨酸积累与H2 O2产生之间的关系，我们在拟南芥中采用药理学和反向遗传学方法，研究了NADPH氧化酶、呼吸爆发氧化酶同源物（Rboh）、研究了200 mM NaCl或400 mM甘露醇胁迫对脯氨酸积累的诱导作用。H2 O2含量的瞬时增加伴随着脯氨酸的积累。二甲基硫脲，一个清除剂的H2 O2，和二苯基碘鎓（DPI），一个抑制剂的H2 O2生产的NADPH氧化酶，被发现显着抑制脯氨酸积累在这些胁迫条件下。DPI还降低了参与脯氨酸生物合成的关键酶Delta（1）-吡咯啉-5-羧酸合成酶的表达水平。同样地，在相同的胁迫下，AtRbohS（A. thaliana Rbohs）在NaCl或甘露醇胁迫下促进H2 O2的产生，从而增加脯氨酸的积累。
Many plants accumulate proline, a compatible osmolyte, in response to various environmental stresses such as water deficit and salinity. In some stress responses, plants generate hydrogen peroxide (H2O2) that mediates numerous physiological and biochemical processes. The aim was to study the relationship between stress-induced proline accumulation and H2O2 production.Using pharmacological and reverse genetic approaches in Arabidopsis thaliana, we investigated the role of NADPH oxidases, Respiratory burst oxidase homologues (Rboh), in the induction of proline accumulation was investigated in response to stress induced by either 200 mM NaCl or 400mM mannitol.Stress from NaCl or mannitol resulted in a transient increase in H2O2 content accompanied by accumulation of proline. Dimethylthiourea, a scavenger of H2O2, and diphenylene iodonium (DPI), an inhibitor of H2O2 production by NADPH oxidase, were found to significantly inhibit proline accumulation in these stress conditions. DPI also reduced the expression level of Delta(1)-pyrroline-5-carboxylate synthetase, the key enzyme involved in the biosynthesis of proline. Similarly, less proline accumulated in knockout mutants lacking either AtRbohD or AtRbohF than in wild-type plants in response to the same stresses.Our data demonstrate that AtRbohs (A. thaliana Rbohs) contribute to H2O2 production in response to NaCl or mannitol stress to increase proline accumulation in this plant.