Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.
Identification of specific corrinoids reveals corrinoid modification in dechlorinating microbial communities.
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DOI：
10.1111/1462-2920.12500
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发表时间：
2015-12
影响因子：
5.1
通讯作者：
Alvarez-Cohen L
中科院分区：
文献类型：
--
作者：
Men Y;Seth EC;Yi S;Crofts TS;Allen RH;Taga ME;Alvarez-Cohen L
钴胺素和其他类咕啉是许多生物体的必需辅因子。大多数具有类咕啉依赖性酶的微生物不会从头产生类咕啉，而是必须获得由其环境中的其他生物产生的类咕啉。然而，在依赖于类咕啉的微生物群落中产生的类咕啉的概况，以及群落成员之间的类咕啉的交换和修饰还没有得到很好的研究。在这项研究中，我们采用了一种新开发的LC/MS/MS为基础的corrinoid检测方法，研究corrinoid，其较低的配体碱，和特定的微生物类群之间的关系，在微生物群落中含有Dehalococcoides mccartyi，具有专性要求苯并咪唑含corrinoid三氯乙烯呼吸。我们发现，对甲酚甲酰胺（[p-Cre]Cba）和钴胺素是最丰富的类可啉的社区。这表明韦荣球菌科的成员与[p-Cre]Cba的产生有关。培养上清中[p-Cre]Cba含量的降低和生物量中钴胺素含量的增加与D.通过脱氯进行麦卡蒂。这支持了D. mccartyi能够通过类咕啉重塑来满足其在群落中的类咕啉需求，在这种情况下，通过输入细胞外[p-Cre]Cba和5，6-二甲基苯并咪唑（DMB）（钴胺素的低级配体）来产生钴胺素作为脱氯的辅因子。这项研究还强调了DMB的作用，在所有研究的社区中产生的较低配体，在corrinoid重塑。这些研究结果提供了新的见解所发挥的作用，不同的系统发育组在微生物群落中的corrinoid生产和corrinoid交换。这项研究也可能有影响，优化氯化溶剂生物修复。
Cobalamin and other corrinoids are essential cofactors for many organisms. The majority of microbes with corrinoid-dependent enzymes do not produce corrinoids de novo, and instead must acquire corrinoids produced by other organisms in their environment. However, the profile of corrinoids produced in corrinoid-dependent microbial communities, as well as the exchange and modification of corrinoids among community members have not been well studied. In this study, we applied a newly developed LC/MS/MS–based corrinoid detection method to examine relationships between corrinoids, their lower ligand bases, and specific microbial groups in microbial communities containing Dehalococcoides mccartyi that has an obligate requirement for benzimidazole-containing corrinoids for trichloroethene-respiration. We found that p-cresolylcobamide ([p-Cre]Cba) and cobalamin were the most abundant corrinoids in the communities. It suggests that members of the family Veillonellaceae are associated with the production of [p-Cre]Cba. The decrease of supernatant-associated [p-Cre]Cba and the increase of biomass-associated cobalamin were correlated with the growth of D. mccartyi by dechlorination. This supports the hypothesis that D. mccartyi is capable of fulfilling its corrinoid requirements in community through corrinoid remodeling, in this case, by importing extracellular [p-Cre]Cba and 5,6-dimethylbenzimidazole (DMB) (the lower ligand of cobalamin), to produce cobalamin as a cofactor for dechlorination. This study also highlights the role of DMB, the lower ligand produced in all of the studied communities, in corrinoid remodeling. These findings provide novel insights on roles played by different phylogenetic groups in corrinoid production and corrinoid exchange within microbial communities. This study may also have implications for optimizing chlorinated solvent bioremediation.