Combinatorial effects of antibiotics and enzymes against dual-speciesStaphylococcus aureusandPseudomonas aeruginosabiofilms in the wound-like medium
Combinatorial effects of antibiotics and enzymes against dual-speciesStaphylococcus aureusandPseudomonas aeruginosabiofilms in the wound-like medium
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DOI：
10.1371/journal.pone.0235093
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发表时间：
2020-06-25
期刊：
影响因子：
3.7
通讯作者：
Jabalameli, Fereshteh
中科院分区：
文献类型：
--
作者：
Fanaei Pirlar, Rima;Emaneini, Mohammad;Jabalameli, Fereshteh
细菌生物膜是治疗慢性感染如慢性伤口的主要问题之一，其中生物膜通常是多微生物的。物种之间的协同作用可以发生在大多数多微生物感染期间，结果是抗菌素耐药性增强。此外，生物膜中的自产细胞外聚合物（EPS）导致对抗生素的高耐受性，这使伤口愈合复杂化。由于大多数抗生素不能清除慢性感染中的生物膜，因此可能需要新的治疗方法。破坏EPS是根除生物被膜的有效途径之一。因此，使用酶降解EPS可导致改善的慢性伤口愈合。在目前的研究中，我们研究了胰蛋白酶，β-葡萄糖苷酶，和DNA酶I酶的双物种生物膜的降解铜绿假单胞菌和金黄色葡萄球菌在伤口样介质的效力。这些菌种是与慢性伤口中生物膜形成相关的两种最常见的细菌。此外，当与酶组合时，评估美罗培南和阿米卡星的最小生物膜根除浓度（MBEC）的降低。胰蛋白酶、β-葡糖苷酶和DNA酶I降解生物膜的最小有效浓度分别为1 μ g/ml、8 U/ml和150 U/ml。0.15 μ g/ml胰蛋白酶和50 U/ml DNA酶I的组合对金黄色葡萄球菌-铜绿假单胞菌生物膜有显著影响，导致所有生物膜的分散和溶解。在酶混合物的存在下，抗生素的MBEC显示出显著降低（p
Bacterial biofilms are one of the major issues in the treatment of chronic infections such as chronic wounds, where biofilms are typically polymicrobial. The synergy between species can occur during most polymicrobial infections, where antimicrobial resistance enhances as a result. Furthermore, self-produced extracellular polymeric substance (EPS) in biofilms results in a high tolerance to antibiotics that complicates wound healing. Since most antibiotics fail to remove biofilms in chronic infections, new therapeutic modalities may be required. Disruption of EPS is one of the effective approaches for biofilm eradication. Therefore, degradation of EPS using enzymes may result in improved chronic wounds healing. In the current study, we investigated the efficacy of trypsin, beta-glucosidase, and DNase I enzymes on the degradation of dual-species biofilms ofPseudomonas aeruginosaandStaphylococcus aureusin a wound-like medium. These species are the two most common bacteria associated with biofilm formation in chronic wounds. Moreover, the reduction of minimum biofilm eradication concentration (MBEC) of meropenem and amikacin was evaluated when combined with enzymes. The minimum effective concentrations of trypsin, beta-glucosidase, and DNase I enzymes to degrade biofilms were 1 mu g/ml, 8 U/ml, and 150 U/ml, respectively. Combination of 0.15 mu g/ml trypsin and 50 U/ml DNase I had a significant effect onS.aureus-P.aeruginosabiofilms which resulted in the dispersal and dissolution of all biofilms. In the presence of the enzymatic mixture, MBECs of antibiotics showed a significant decrease (p