A random arrangement of albumin‐containing hepatocytes seen with histo‐immunologic methods. II. Conditions that produce the artifact

A random arrangement of albumin‐containing hepatocytes seen with histo‐immunologic methods. II. Conditions that produce the artifact
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用组织免疫学方法观察到的含白蛋白肝细胞的随机排列。

DOI:
10.1002/ar.1091970208
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发表时间:
1980
期刊:
The Anatomical Record
影响因子:
--
通讯作者:
J. Peters Masse
J. Peters Masse
中科院分区:
--
文献类型:
--
作者:
A. LeBouton;J. Peters Masse

文献摘要

被引文献

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大鼠肝脏中白蛋白的细胞免疫定位已被研究为各种生理和物理条件的函数。我们的观察表明,白蛋白和其他肝蛋白准确免疫定位的首要条件是在固定之前从血窦和迪斯窦周间隙中完全去除血液,尤其是血浆。
The cellular immunolocalization of albumin in rat liver has been studied as a function of various physiological and physical conditions. Our observations show that the prime requisite for accurate immunolocalization of albumin and other hepatic‐based proteins is the complete removal of blood and especially plasma from sinusoids and the perisinusoidal space of Disse prior to fixation.