Potential indicators of stress response identified by expressed sequence tag analysis of hemocytes and embryos from the American oyster, Crassostrea virginica
Potential indicators of stress response identified by expressed sequence tag analysis of hemocytes and embryos from the American oyster, Crassostrea virginica
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DOI：
10.1007/s10126-001-0072-8
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发表时间：
2002-01-01
影响因子：
3
通讯作者：
Chapman, RW
中科院分区：
文献类型：
--
作者：
Jenny, MJ;Ringwood, AH;Chapman, RW
一个试点计划启动，以确定基因从美国牡蛎，Crassostrea virginica，这是潜在的参与应激反应作为生物指标暴露于环境污染物和有毒和传染性病原体。一个基于PCR的方法被用来构建从合并的胚胎和单个个体的血细胞的cDNA文库。共998个随机选择的克隆（表达序列标签，EST）进行测序。大约40%的EST是新序列。几个潜在的生物标志物包括抗菌肽，识别分子（凝集素受体），蛋白酶和蛋白酶抑制剂，和一种新的金属硫蛋白。多样性分析表明，363和286个独特的基因，分别从血细胞和胚胎库，表明全面的EST收集是一个有价值的方法，发现新的基因的潜在意义，在软体动物的压力反应。
A pilot program was initiated to identify genes from the American oyster, Crassostrea virginica, that are potentially involved in the stress response for use as bioindicators of exposure to environmental pollutants and to toxic and infectious agents. A PCR-based method was used to construct cDNA libraries from pooled embryos and the hemocytes of a single individual. A total of 998 randomly selected clones (expressed sequence tags, ESTs) were sequenced. Approximately 40% of the ESTs are novel sequences. Several potential biomarkers identified include an antimicrobial peptide, recognition molecules (lectin receptors), proteinases and proteinase inhibitors, and a novel metallothionein. Diversity analysis shows that 363 and 286 unique genes were identified from the hemocyte and embryo libraries, respectively, indicating that full-scale EST collection is a valuable approach for the discovery of new genes of potential significance in the molluscan stress response.