Activation of focal adhesion kinase induces extracellular signal-regulated kinase-mediated osteogenesis in tensile force-subjected periodontal ligament fibroblasts but not in osteoblasts
Activation of focal adhesion kinase induces extracellular signal-regulated kinase-mediated osteogenesis in tensile force-subjected periodontal ligament fibroblasts but not in osteoblasts
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DOI：
10.1007/s00774-013-0549-3
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发表时间：
2014-11-01
影响因子：
3.3
通讯作者：
Kao, Chia-Tze
中科院分区：
文献类型：
--
作者：
Chen, Yi-Jyun;Shie, Ming-You;Kao, Chia-Tze
粘着斑激酶（FAK）在受力细胞中将机械信号转化为成骨分化的确切机制尚未阐明。对不同力的反应根据细胞的来源和施加的应力类型而不同。因此，破骨细胞和成骨细胞祖细胞的募集，以及牙周膜（PDL）周围和内部这些细胞的平衡激活对于牙槽骨重建至关重要。PDL和MG 63细胞内的细胞经受-100 kPa的张力持续不同的时间段。在施加张力期间的不同时间，对其进行处理，以分析细胞活力、细胞周期和成骨蛋白。同时观察FAK靶向小干扰RNA转染的效果。张力促进了PDL细胞FAK磷酸化的快速增加和成骨蛋白表达的上调，但在MG 63细胞中没有。FAK反义寡核苷酸转染PDL细胞后，碱性磷酸酶和骨钙素分泌减少。这些发现表明，张力激活PDL细胞中的FAK通路，其下调免疫细胞因子并上调成骨蛋白。
The exact mechanism by which focal adhesion kinase (FAK) translates mechanical signals into osteogenesis differentiation in force-subjected cells has not been elucidated. The responses to different forces differ according to the origin of cells and the type of stress applied. Therefore, the recruitment of osteoclast and osteoblast progenitor cells, and the balanced activation of these cells around and within the periodontal ligament (PDL) are essential for alveolar bone remodeling. Cells within the PDL and MG63 cells were subjected to tensile forces of - 100 kPa for different periods of time. At various times during the tensile force application, they were processed for the purpose of analyzing cell viability, cell cycle, and osteogenic protein. The effect of small interfering RNA transfection targeting FAK was also evaluated. Tensile force enhanced a rapid increase in the phosphorylation of FAK and up-regulated osteogenic protein expression in PDL cells, but not in MG63 cells. Transfecting PDL cells with FAK antisense oligonucleotide diminished alkaline phosphatase and osteocalcin secretion. These findings suggest that tensile force activates FAK pathways in PDL cells, which down-regulate immune cytokine and up-regulate osteogenic protein.