Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa.
Phenazine production enhances extracellular DNA release via hydrogen peroxide generation in Pseudomonas aeruginosa.
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DOI：
10.4161/cib.23570
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发表时间：
2013-05-01
影响因子：
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通讯作者：
Manefield M
中科院分区：
文献类型：
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作者：
Das T;Manefield M
在铜绿假单胞菌中，eDNA是生物膜形成和稳定所必需的关键成分。在这项研究中，我们报告说，释放eDNA的影响，生产吩嗪铜绿假单胞菌。与产生吩嗪的菌株相比，吩嗪产生缺陷的铜绿假单胞菌PA 14的A-G突变体产生显著更少的eDNA。eDNA释放和吩嗪产生之间的关系通过过氧化氢（H2 O2）产生和随后的H2 O2介导的细胞裂解以及最终将染色体DNA作为eDNA释放到细胞外环境中来桥接。
In Pseudomonas aeruginosa eDNA is a crucial component essential for biofilm formation and stability. In this study we report that release of eDNA is influenced by the production of phenazine in P. aeruginosa. A ∆phzA-G mutant of P. aeruginosa PA14 deficient in phenazine production generated significantly less eDNA in comparison with the phenazine producing strains. The relationship between eDNA release and phenazine production is bridged via hydrogen peroxide (H2O2) generation and subsequent H2O2 mediated cell lysis and ultimately release of chromosomal DNA into the extracellular environment as eDNA.