Tissue-specific mRNA expression profiles of human solute carrier transporter superfamilies
Tissue-specific mRNA expression profiles of human solute carrier transporter superfamilies
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DOI：
10.2133/dmpk.23.22
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Naito, Shinsaku
中科院分区：
文献类型：
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作者：
Nishimura, Masuhiro;Naito, Shinsaku
制备了173个人溶质载体(SLC)转运蛋白的正反向引物和TaqMan探针。采用实时逆转录聚合酶链式反应(RT-PCR)技术，分析不同组织(肾上腺、膀胱、骨髓、脑、结肠、心脏、肾脏、肝、肺、乳腺、卵巢、胰腺、外周血白细胞、胎盘、前列腺、视网膜、唾液腺、骨骼肌、小肠、平滑肌、脊髓、脾、胃、睾丸、胸腺、甲状腺、气管和子宫)单个和混合标本中各靶转运蛋白的mRNA表达水平。人类SLC转运蛋白在肝脏中表达的个体差异也被评估。这些新确定的表达谱被用来研究上述28种不同的人类组织中的基因表达，并找到了对人类SLC转运蛋白具有高转录活性的组织。这些结果有望对疾病的临床诊断研究有价值。
Pairs of forward and reverse primers and TaqMan probes specific to each of 173 human solute carrier (SLC) transporters were prepared. The mRNA expression level of each target transporter was analyzed in total RNA from single and pooled specimens of various human tissues (adrenal gland, bladder, bone marrow, brain, colon, heart, kidney, liver, lung, mammary gland, ovary, pancreas, peripheral leukocytes, placenta, prostate, retina, salivary gland, skeletal muscle, small intestine, smooth muscle, spinal cord, spleen, stomach, testis, thymus, thyroid gland, trachea, and uterus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. Individual differences in the mRNA expression of human SLC transporters in the liver were also evaluated. These newly determined expression profiles were used to study the gene expression in the 28 different human tissues listed above, and tissues with high transcriptional activity for human SLC transporters were identified. These results are expected to be valuable for research concerning the clinical diagnosis of disease.