The mechanochemistry of integrated motor protein complexes
The mechanochemistry of integrated motor protein complexes
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DOI：
10.1016/j.jbiomech.2009.09.006
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发表时间：
2010-01-05
影响因子：
2.4
通讯作者：
Diehl, Michael R.
中科院分区：
文献类型：
--
作者：
Constantinou, Pamela E.;Diehl, Michael R.
分子马达蛋白组装成多单位蛋白质复合物在决定许多亚细胞商品的细胞内转运和运输特性中起着重要作用。然而，尚不清楚这些复合物中的蛋白质如何相互作用并共同发挥作用。考虑到汽车运输和疾病之间的联系，它已成为越来越重要的调查这些基本的运输“图案”的功能特性。这样做需要表征多单元电机组件的复合运动和力产生特性。然而，这样的分析通常是混淆了缺乏了解的许多运动复合体的结构和机械性能之间的联系。新的实验挑战也出现时，一个检查运动合作。为了充分理解多电机复合体的传输行为，必须检查电机合奏可用的机械微观状态的分布。此外，马达通信的机制必须加以探讨，以确定马达组是否可以真正合作的方式一起移动货物。解决这些问题需要开发实验方法，使运输蛋白质的复杂系统的动力学进行监测与单分子生物物理测定相同的精度。在这里，我们讨论了关键的基本原则，管理电机复合体的功能和它们的关系，调节细胞内货物运输的机制。我们还概述了新的实验策略，以解决这些细胞内运输的基本特征。爱思唯尔有限公司出版
The assembly of molecular motor proteins into multi-unit protein complexes plays an important role in determining the intracellular transport and trafficking properties of many subcellular commodities. Yet, it is not known how proteins within these complexes interact and function collectively. Considering the established ties between motor transport and diseases, it has become increasingly important to investigate the functional properties of these essential transport 'motifs'. Doing so requires that the composite motile and force-generating properties of multi-unit motor assemblies are characterized. However, such analyses are typically confounded by a lack of understanding of the links between the structural and mechanical properties of many motor complexes. New experimental challenges also emerge when one examines motor cooperation. Distributions in the mechanical microstates available to motor ensembles must be examined in order to fully understand the transport behavior of multi-motor complexes. Furthermore, mechanisms by which motors communicate must be explored to determine whether motor groups can move cargo together in a truly cooperative fashion. Resolving these issues requires the development of experimental methods that allow the dynamics of complex systems of transport proteins to be monitored with the same precision available to single-molecule biophysical assays. Herein, we discuss key fundamental principles governing the function of motor complexes and their relation to mechanisms that regulate intracellular cargo transport. We also outline new experimental strategies to resolve these essential features of intracellular transport. Published by Elsevier Ltd.