TYR527 IS PHOSPHORYLATED IN PP60C-SRC - IMPLICATIONS FOR REGULATION
TYR527 IS PHOSPHORYLATED IN PP60C-SRC - IMPLICATIONS FOR REGULATION
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DOI：
10.1126/science.2420005
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发表时间：
1986-03-21
期刊：
影响因子：
56.9
通讯作者：
HUNTER, T
中科院分区：
文献类型：
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作者：
COOPER, JA;GOULD, KL;HUNTER, T
劳斯肉瘤病毒癌基因产物pp 60 v-src可转化培养的成纤维细胞，但其相应的原癌基因产物pp 60 c-src则不能。已知这两种蛋白质都是蛋白质酪氨酸激酶。已发表的结果表明，相对于pp 60 v-src，pp 60 c-src的激酶活性被抑制，这可能是由于pp 60 v-src中没有磷酸化的pp 60 c-src中的酪氨酸的磷酸化。在本研究中，观察到pp 60 c-src中磷酸化的酪氨酸是Tyr 527，距离蛋白质的COOH-末端6个残基。pp 60 c-src从残基515到COOH-末端的区域，包括Tyr 527，已被pp 60 v-src中的不同序列取代。因此，在pp 60 v-src的形成中发生的转化能力和激酶活性的增加可能是由于参与负调节的酪氨酸的损失。
The Rous sarcoma virus oncogene product, pp60v-src, transforms cultured fibroblasts but its corresponding proto-oncogene product, pp60c-src, does not. Both proteins are known to be protein-tyrosine kinases. Published results suggest that the kinase activity of pp60c-src is inhibited relative to that of pp60v-src, due perhaps to phosphorylation of a tyrosine in pp60c-src that is not phosphorylated in pp60v-src. In this study, it was observed that the tyrosine phosphorylated in pp60c-src is Tyr527, six residues from the COOH-terminus of the protein. The region of pp60c-src from residue 515 to the COOH-terminus, including Tyr527, has been replaced with a different sequence in pp60v-src. Thus, the increase in transforming ability and kinase activity that occurred in the genesis of pp60v-src may have resulted from the loss of a tyrosine involved in negative regulation.