Physiological characters of Sporothrix schenckii isolates
Physiological characters of Sporothrix schenckii isolates
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DOI：
10.1046/j.1439-0507.2002.d01-121.x
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发表时间：
2002-12-01
期刊：
影响因子：
4.9
通讯作者：
Chakrabarti, A
中科院分区：
文献类型：
--
作者：
Ghosh, A;Maity, PK;Chakrabarti, A
孢子虫病在印度的三个地区（东部、北部和南部）流行。对来自这三个地区的49株临床分离株（25株来自印度北部，17株来自印度东部，7株来自印度南部）进行了菌丝和酵母形态的殖民地形态和生理特性分析。49个菌株在3种不同培养基上的菌落特征无差异。所有菌株的生长在40 ℃时都受到抑制。发现酵母形式对渗透压和盐浓度更耐受。大多数菌丝体形式在pH 3-12.0之间生长良好，而大多数酵母形式可以耐受2.4至9.5的pH范围。不同地理区域的菌株对阿拉伯糖、糊精、棉子糖、鼠李糖和淀粉的同化能力存在差异。酵母形态不显示任何尿素酶活性，但所有菌株的菌丝形态都能分解尿素。所有菌株的酚氧化酶和硝酸钾同化活性均为阳性，明胶酶活性和酪蛋白水解活性均为阴性。
Sporotrichosis is endemic in three regions (east, north and south) in India. The colony morphology and physiological characteristics of 49 clinical isolates from these three regions (25 from north India, 17 from east India and seven from south India were analysed in both mycelial and yeast forms. No difference in colony character was seen among the 49 isolates on three different media. Growth of all isolates was inhibited at 40 degreesC. The yeast forms were found to be more tolerant to osmotic pressure, and salt concentrations. Most mycelial forms grew well between pH 3-12.0 whereas most yeast forms could tolerate a pH range of 2.4 to 9.5. Variations in assimilation of arabinose, dextrin, raffinose, rhamnose and starch was observed among strains from different geographical regions. The yeast forms did not show any urease activity but the mycelial forms of all isolates could split urea. Phenol oxidase and potassium nitrate assimilation were positive and gelatinase activity and casein hydrolysis were negative for all isolates.