Factors associated with the adherence and biofilm formation by Aeromonas caviae on glass surfaces
Factors associated with the adherence and biofilm formation by Aeromonas caviae on glass surfaces
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DOI：
10.1046/j.1365-2672.2003.01931.x
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发表时间：
2003-01-01
影响因子：
4
通讯作者：
Blondeau, R
中科院分区：
文献类型：
--
作者：
Béchet, M;Blondeau, R
目的：了解豚鼠气单胞菌促进表面生物膜形成的因素。方法与结果：在营养发酵液中，豚鼠气单胞菌LMG-13455能够在玻璃瓶表面形成生物膜，但这种生物膜的形成不一致。这种菌株的一个衍生物，称为M12，促进了可靠和强烈附着的生物膜的快速形成，大约一半的细胞被附着。与亲本相比，M12具有疏水性，在液体介质中表现为自聚集，合成的多糖很少，在游动和聚集运动方面存在缺陷，并在运动软琼脂上出现了特有的表型。进一步的分析表明，这些特性中的大多数与IV型菌毛的存在导致细胞肥大有关，并暗示了一种遗传变异机制，通过改变运动附属物的性质，允许变异细菌附着在惰性表面上。结论：M12是亲本菌株的一个稳定变体，通过IV型菌毛促进强烈附着的生物膜。研究的意义和影响：野生型猪瘟杆菌LMG 13455包括可能与其对不同环境变化的适应有关的亚群。
Aims: Knowledge on factors of Aeromonas caviae promoting the formation of biofilms on surfaces.Methods and Results: In nutrient broth under agitation, A. caviae LMG 13455 was able to form biofilms on the surfaces of glass flasks, but such biofilm formation was inconsistent. A derivative of this strain, called M12, promoted the rapid formation of reliable and strongly adherent biofilms with about half of the cells being adhered. In contrast with its parent, M12 was hydrophobic, displayed auto-aggregation in liquid medium, synthesized very little polysaccharides and was defective in swimming and swarming motilities, together with the appearence of a characteristic phenotype on motility soft agar. Further analyses demonstrated that most of these properties were related to a hyperpiliation of the cells through the presence of type IV pili, and suggested that a mechanism of genetic variation, by altering the nature of motility appendages, allows the variant bacteria to attach on inert surfaces.Conclusions: M12 is a stabilized variant of the parental strain, promoting strongly adherent biofilms through the type IV pili.Significance and Impact of the Study: The wild-type strain A. caviae LMG 13455 include subpopulations that are likely implicated in its adaptation to different environmental changes.