Endothelial cell survival on transplanted human corneas preserved by organ culture with 1.35% chondroitin sulfate.
Endothelial cell survival on transplanted human corneas preserved by organ culture with 1.35% chondroitin sulfate.
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DOI：
10.1016/s0002-9394(14)73368-9
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发表时间：
1985-12
影响因子：
4.2
通讯作者：
W. M. Bourne;R. Lindstrom;D. Doughman
中科院分区：
文献类型：
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作者：
W. M. Bourne;R. Lindstrom;D. Doughman
我们将30个供体角膜在含1.35%硫酸软骨素的培养基中于34 ℃保存15 ~ 33天后进行移植，并将它们与同期移植的30个角膜进行比较，但仅在McCarey-Kaufman培养基中于4 ℃保存1 ~ 81小时。角膜移植术后两个月，器官培养的移植物和保存在McCarey-Kaufman培养基中的移植物之间的中央内皮细胞损失没有统计学显著差异（分别为9%和7%细胞损失）。该研究在90%功率下检测到约10%或更多的细胞损失差异。术后第1天，器官培养的角膜移植片较McCarey-Kaufman培养液保存的角膜移植片厚，培养时间最长的角膜移植片最厚。3周后两组角膜厚度相似。这些结果表明，供体角膜内皮细胞在34 ℃下在1.35%硫酸软骨素中保存长达一个月似乎在角膜移植术后存活并发挥功能，以及那些在4 ℃下在McCarey-Kaufman培养基中保存1 - 3天的角膜内皮细胞。
We transplanted 30 donor corneas preserved at 34 C for 15 to 33 days in culture medium containing 1.35% chondroitin sulfate and compared them with 30 corneas transplanted during the same period, but preserved only in McCarey-Kaufman medium at 4 C for one to 81 hours. Two months after keratoplasty there was no statistically significant difference in central endothelial cell loss between the organ-cultured grafts and those preserved in McCarey-Kaufman medium (9% vs 7% cell loss, respectively). The study detected with 90% power a difference in cell loss of approximately 10% or more. On the first postoperative day, the organ-cultured grafts were thicker than those grafts preserved in McCarey-Kaufman medium, and the thickest corneas were those cultured for the longest times. The corneal thickness was similar in the two groups after three weeks. These results demonstrated that donor corneal endothelial cells preserved for up to one month at 34 C in 1.35% chondroitin sulfate appear to survive and function after keratoplasty as well as those preserved at 4 C for one to three days in McCarey-Kaufman medium.