SEPARATION OF HUMAN EPIDERMAL STEM-CELLS FROM TRANSIT AMPLIFYING CELLS ON THE BASIS OF DIFFERENCES IN INTEGRIN FUNCTION AND EXPRESSION
SEPARATION OF HUMAN EPIDERMAL STEM-CELLS FROM TRANSIT AMPLIFYING CELLS ON THE BASIS OF DIFFERENCES IN INTEGRIN FUNCTION AND EXPRESSION
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DOI：
10.1016/0092-8674(93)90251-k
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发表时间：
1993-05-21
期刊：
影响因子：
64.5
通讯作者：
WATT, FM
中科院分区：
文献类型：
--
作者：
JONES, PH;WATT, FM
表皮被认为含有两种类型的增殖细胞：干细胞和具有较低自我更新能力和较高终末分化概率的细胞（转运扩增细胞）。我们报告说，角质形成细胞的干细胞的特性，可以从培养的人表皮的基础上，高表面表达的β 1整合素和快速粘附细胞外基质（ECM）蛋白分离。在角质形成细胞中，β 1整合素在细胞表面上的相对水平与增殖能力之间存在对数线性关系;此外，具有最高集落形成效率的细胞最迅速地粘附于IV型胶原蛋白、纤连蛋白或角质形成细胞ECM。粘附较慢的增殖角质形成细胞具有过境扩增细胞的特征：经过1至5轮分裂后，它们的所有子细胞都经历了终末分化。由于干细胞可以根据其粘附特性分离到大于90%的纯度，因此现在可以研究调节其后代命运的机制。
The epidermis is believed to contain two types of proliferating cells: stem cells and cells with a lower capacity for self-renewal and higher probability of undergoing terminal differentiation (transit amplifying cells). We report that keratinocytes with characteristics of stem cells can be isolated from cultured human epidermis on the basis of high surface expression of beta1 integrins and rapid adhesion to extracellular matrix (ECM) proteins. Among keratinocytes there was a log linear relationship between the relative level of beta1 integrins on the cell surface and proliferative capacity; furthermore, the cells with the highest colony-forming efficiency adhered most rapidly to type IV collagen, fibronectin, or keratinocyte ECM. Proliferating keratinocytes that adhered more slowly had characteristics of transit amplifying cells: after one to five rounds of division, all of their daughters underwent terminal differentiation. Since stem cells can be isolated to greater than 90% purity on the basis of their adhesive properties, it will now be possible to investigate the mechanisms that regulate the fate of their progeny.