The sensilla trichodea-biased EoblPBP1 binds sex pheromones and green leaf volatiles in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones
The sensilla trichodea-biased EoblPBP1 binds sex pheromones and green leaf volatiles in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones
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Ectropis obliqua Prout（一种使用 II 型性信息素的尺蛾害虫）中的毛形感器 EoblPBP1 与性信息素和绿叶挥发物结合
DOI：
10.1016/j.jinsphys.2019.04.005
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发表时间：
2019-07-01
影响因子：
2.2
通讯作者：
Xiao, Qiang
中科院分区：
文献类型：
--
作者：
Sun, Liang;Wang, Qian;Xiao, Qiang
信息素结合蛋白（PBP）被认为在性信息素检测中起着关键作用。鳞翅目蛾可分为两个分类群，使用I型性信息素的那些，如C10-C18不饱和醛、醇和乙酸酯，和使用II型信息素的那些，其为C17-C23多不饱和烃及其环氧化物衍生物。迄今为止，几乎所有的特征性PBP已被报道在蛾类与I型性信息素，和PBP的生理功能，使用II型性信息素的蛾仍不清楚。在本研究中，我们功能性研究EoblPBP 1在Ectropis的Ectropis的Prout，一个重要的尺蠖蛾害虫，使用II型性信息素。系统发育分析表明，EoblPBP 1与尺蠖Ⅱ型性信息素检测基因ScerPBP 1聚在一起。扫描电子显微镜显示E.雌雄蛾的触角感器主要有6种类型，分别为Str-Ⅰ和Str-Ⅱ两种类型的触角感器、锥形感器（Sba）、柱锥形感器（Sst）、刺形感器（Sch）和耳形感器（Sau）。其中，Str-I被证实是雄性蛾特异性的，并且具有五种不同的亚型。荧光原位杂交结果显示EoblPBP 1主要表达于Str-I的碱基。比较结合试验表明，重组EoblPBP 1与E.这表明它参与了II型性信息素的检测。此外，EobIPBP 1还高度结合不饱和乙酸信息素和绿色叶挥发物。这些结果表明PBP 1与检测E.但不能区分II型性信息素与I型性信息素或绿色叶挥发物。本研究结果为进一步研究鳞翅目蛾Ⅱ型性信息素识别的分子基础奠定了基础。
Pheromone-binding proteins (PBPs) are considered to play critical roles in sex pheromone detection. Lepidopteran moths can be divided into two taxa, those that use Type-I sex pheromones, such as C10-C18 unsaturated aldehydes, alcohols and acetates, and those that use Type-II pheromones, which are C17-C23 polyunsaturated hydrocarbons and their epoxide derivatives. To date, nearly all the characterized PBPs have been reported in moths with Type-I sex pheromones, and the physiological functions of PBPs in moths that use Type-II sex pheromones remains unclear. In the present study we functionally examine EoblPBP1 in Ectropis obliqua Prout, an important geometrid moth pest that uses Type-II sex pheromones. The phylogenetic analysis of the sequence indicated that EoblPBP1 clustered together with ScerPBP1, a geometrid PBP for detecting Type-II sex pheromones. Scanning electron microscopy showed that E. obliqua moths of both sexes mainly had six types of antennal sensilla, including two types of sensilla trichodea, Str-I and Str-II, sensilla basiconica (Sba), sensilla styloconica (Sst), sensilla chaetica (Sch) and sensilla auricillica (Sau). Of these, Str-I was confirmed to be male moth-specific and had five different subtypes. Fluorescence in situ hybridization revealed that EoblPBP1 was primarily expressed at the base of Str-I. A comparative binding assay showed that recombinant EoblPBP1 bound three sex pheromone components of E. obliqua, demonstrating its involvement in the detection of Type-II sex pheromones. Besides, EobIPBP1 also highly bound unsaturated acetates pheromones and the green leaf volatiles. These results indicate that PBP1 is associated with detecting Type-II sex pheromones in E. obliqua but cannot differentiate Type-II sex pheromones from Type-I sex pheromones or green leaf volatiles. Our findings provide a foundation for further study on molecular basis of Type-II sex pheromone recognition in lepidopteran moths.