Dissociation kinetics and equilibrium binding properties of polyene antibiotic complexes with phosphatidylcholine/sterol vesicles.
Dissociation kinetics and equilibrium binding properties of polyene antibiotic complexes with phosphatidylcholine/sterol vesicles.
复制标题
多烯抗生素与磷脂酰胆碱/甾醇囊泡复合物的解离动力学和平衡结合特性。
DOI：
10.1021/bi00303a014
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发表时间：
1984
期刊：
影响因子：
2.9
通讯作者：
Bittman,R
中科院分区：
文献类型：
--
作者：
Witzke,NM;Bittman,R
Niels M. Witzke 和 Robert Bittman* 摘要：在低于多烯发生自缔合的浓度下，通过紫外-可见光谱检查了超声处理囊泡与多烯抗生素两性霉素 B、念珠菌素、mediocidin 以及两性霉素 B 的水溶性胍衍生物的相互作用。在 30°C 和 pH 7.4 下测定缔合常数、Kapp 和每个甾醇或磷脂分子的结合位点数量 (n)。发现了一类结合位点，没有协同作用的证据。对于 mediocidin、两性霉素 B 和胍衍生物与磷脂酰胆碱 (PC)、PC/胆固醇和 PC/麦角甾醇囊泡的结合，
Niels M. Witzke and Robert Bittman* abstract: The interactions of sonicatedvesicles with the polyene antibiotics amphotericin B, candicidin, mediocidin, and a water-soluble, guanidine derivative of amphotericin B were examined by UV-visible spectroscopy at concentrations below which the polyenes become self-associated. The asso-ciation constants, Kapp, and the numbers of binding sites per sterol or phospholipid molecule (n) were determined at 30 C and pH 7.4. A single class of binding sites was found, with no evidence of cooperativity. For the binding of mediocidin, amphotericin B, and the guanidine derivative with phospha-tidylcholine (PC), PC/cholesterol, and PC/ergosterol vesicles,