Comparative transcriptional survey between self-incompatibility and self-compatibility in Citrus reticulata Blanco
Comparative transcriptional survey between self-incompatibility and self-compatibility in Citrus reticulata Blanco
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柑橘自交不亲和性与自交亲和性转录比较研究
DOI：
10.1016/j.gene.2017.01.033
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发表时间：
2017-04-20
期刊：
影响因子：
3.5
通讯作者：
Qin, Yonghua
中科院分区：
文献类型：
--
作者：
Ma, Yuewen;Li, Qiulei;Qin, Yonghua
无核是优良的经济性状，自交不亲和性是造成柑橘无核的重要因素之一。然而，柑橘SI的分子机制仍不清楚。本研究利用RNA-Seq技术筛选了与五子沙汤菊SI反应相关的差异表达基因。总共产生了35.67 GB的原始RNA-Seq数据，并重新组装成50,364个单基因，平均长度为897bp，N50值为1549。对23个与自花授粉和异花授粉后不同组织、不同时期的与SI相关的候选基因进行了qPCR分析。鉴定出7个花粉S基因(Unigene0050323、Unigene0001060、Unigene0004230、Unigene0004222、Unigene0012037、Unigene0048889和Unigene0004272)、3个雌蕊S基因(Unigene0019191、Unigene0040115、Unigene0036542)和3个基因(Unigene0038751、Unigene0031435和Unigene0029897)。根据表达分析，Unigene0031435、Unigene0038751和Unigene0029897可能参与了‘五子莎汤菊’的SI反应。本研究为从转录水平上研究柑橘SI的分子机制提供了新的思路。(C)2017爱思唯尔B.V.保留所有权利。
Seedlessness is an excellent economical trait, and self-incompatibility (SI) is one of important factors resulting in seedless fruit in Citrus. However, SI molecular mechanism in Citrus is still unclear. In this study, RNA-Seq technology was used to identify differentially expressed genes related to SI reaction of'Wuzishatangju' (Citrus reticulata Blanco). A total of 35.67 GB raw RNA-Seq data was generated and was de novo assembled into 50,364 unigenes with an average length of 897 bp and N50 value of 1549. Twenty-three candidate unigenes related to SI were analyzed using qPCR at different tissues and stages after self- and cross-pollination. Seven pollen S genes (Unigene0050323, Unigene0001060, Unigene0004230, Unigene0004222, Unigene0012037, Unigene0048889 and Unigene0004272), three pistil S genes (Unigene0019191, Unigene0040115, Unigene0036542) and three genes (Unigene0038751, Unigene0031435 and Unigene0029897) associated with the pathway of ubiquitin-mediated proteolysis were identified. Unigene0031435, Unigene0038751 and Unigene0029897 are probably involved in SI reaction of'Wuzishatangju' based on expression analyses. The present study provides a new insight into the molecular mechanism of SI in Citrus at the transcriptional level. (C) 2017 Elsevier B.V. All rights reserved.