Nucleator-dependent intercellular assembly of adhesive curli organelles in Escherichia coli
Nucleator-dependent intercellular assembly of adhesive curli organelles in Escherichia coli
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DOI：
10.1073/pnas.93.13.6562
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发表时间：
1996-06-25
影响因子：
11.1
通讯作者：
Normark, S
中科院分区：
文献类型：
--
作者：
Hammar, M;Bian, Z;Normark, S
细菌对其他细菌、真核细胞和细胞外基质蛋白的黏附通常是由由一个或多个亚单位蛋白质组成的细胞表面相关聚合物(菌毛)介导的。我们发现，在大肠杆菌表面，Curlin聚合成类菌毛结构(Curli)与目前流行的菌毛组装模型有明显的不同，因为它是通过依赖于特定核蛋白的细胞外自组装过程发生的。细胞表面结合的核因子启动了由核因子呈递细胞或相邻细胞分泌的Curlin的聚合。在生长的细丝中加入单体似乎是由质量作用驱动的，仅受分泌单体来源和单体凝聚表面之间的扩散梯度的引导。
Bacterial adhesion to other bacteria, to eukaryotic cells, and to extracellular matrix proteins is frequently mediated by cell surface-associated polymers (fimbriae) consisting of one or more subunit proteins. We have found that polymerization of curlin to fimbriae-like structures (curli) on the surface of Escherichia coli markedly differs from the prevailing model for fimbrial assembly in that it occurs extracellularly through a self-assembly process depending on a specific nucleator protein. The cell surface-bound nucleator primes the polymerization of curlin secreted by the nucleator-presenting cell or by adjacent cells. The addition of monomers to the growing filament seems to be driven by mass action and guided only by the diffusion gradient between the source of secreted monomer and the surface of monomer condensation.