Mouse adult hematopoietic stem cells actively synthesize ribosomal RNA.
Mouse adult hematopoietic stem cells actively synthesize ribosomal RNA.
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DOI：
10.1261/rna.067843.118
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发表时间：
2018-12
期刊：
影响因子：
--
通讯作者：
Cohen-Tannoudji M
中科院分区：
文献类型：
--
作者：
Jarzebowski L;Le Bouteiller M;Coqueran S;Raveux A;Vandormael-Pournin S;David A;Cumano A;Cohen-Tannoudji M
基础细胞过程对组织稳态调节的贡献才刚刚开始受到重视。然而，我们对特定谱系内核糖体生物合成活性原位调节的了解仍然非常有限。这在很大程度上是由于缺乏能够在体内少量细胞中定量核糖体生物合成的测定。我们使用了一种技术，命名为Flow-FISH，结合细胞表面抗体染色和流式细胞术与细胞内核糖体RNA（rRNA）FISH，测量体内造血细胞的pre-rRNA水平。在这里，我们表明Flow-FISH报告并量化了造血细胞群体中的核糖体生物合成活性，从而提供了关于这一基本过程的原始数据，特别是在罕见的群体中，如造血干细胞和祖细胞。我们揭示了不同造血祖细胞间室和红系分化过程中前rRNA水平的变化。特别是，我们的数据表明，相反，可以从他们的静止状态预期，造血干细胞具有显着的核糖体生物合成活性。此外，前rRNA水平的变化与增殖率无关，表明细胞类型特异性机制可能调节造血干细胞和祖细胞中的核糖体生物合成。我们的研究有助于更好地了解造血系统的细胞生理学在体内不受干扰的情况。
The contribution of basal cellular processes to the regulation of tissue homeostasis has just started to be appreciated. However, our knowledge of the modulation of ribosome biogenesis activity in situ within specific lineages remains very limited. This is largely due to the lack of assays that enable quantitation of ribosome biogenesis in small numbers of cells in vivo. We used a technique, named Flow-FISH, combining cell surface antibody staining and flow cytometry with intracellular ribosomal RNA (rRNA) FISH, to measure the levels of pre-rRNAs of hematopoietic cells in vivo. Here, we show that Flow-FISH reports and quantifies ribosome biogenesis activity in hematopoietic cell populations, thereby providing original data on this fundamental process notably in rare populations such as hematopoietic stem and progenitor cells. We unravel variations in pre-rRNA levels between different hematopoietic progenitor compartments and during erythroid differentiation. In particular, our data indicate that, contrary to what may be anticipated from their quiescent state, hematopoietic stem cells have significant ribosome biogenesis activity. Moreover, variations in pre-rRNA levels do not correlate with proliferation rates, suggesting that cell type-specific mechanisms might regulate ribosome biogenesis in hematopoietic stem cells and progenitors. Our study contributes to a better understanding of the cellular physiology of the hematopoietic system in vivo in unperturbed situations.
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影响因子：
64.5
作者：
Buszczak M;Signer RA;Morrison SJ
通讯作者：
Morrison SJ
影响因子：
3.7
作者：
Hayashi Y;Kuroda T;Kishimoto H;Wang C;Iwama A;Kimura K
通讯作者：
Kimura K
影响因子：
64.8
作者：
Busch, Katrin;Klapproth, Kay;Rodewald, Hans-Reimer
通讯作者：
Rodewald, Hans-Reimer
DOI：
10.1073/pnas.1418845112
发表时间：
2015-02-17
影响因子：
11.1
作者：
Fortier, Simon;MacRae, Tara;Sauvageau, Guy
通讯作者：
Sauvageau, Guy
抑制RNA聚合酶I作为促进p53癌症特异性激活的治疗策略。
影响因子：
50.3
作者：
Bywater MJ;Poortinga G;Sanij E;Hein N;Peck A;Cullinane C;Wall M;Cluse L;Drygin D;Anderes K;Huser N;Proffitt C;Bliesath J;Haddach M;Schwaebe MK;Ryckman DM;Rice WG;Schmitt C;Lowe SW;Johnstone RW;Pearson RB;McArthur GA;Hannan RD
通讯作者：
Hannan RD