High-Resolution Genotyping of Wild Barley Introgression Lines and Fine-Mapping of the Threshability Locus thresh-1 Using the Illumina GoldenGate Assay.
High-Resolution Genotyping of Wild Barley Introgression Lines and Fine-Mapping of the Threshability Locus thresh-1 Using the Illumina GoldenGate Assay.
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DOI：
10.1534/g3.111.000182
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发表时间：
2011-08
期刊：
影响因子：
--
通讯作者：
Pillen K
中科院分区：
文献类型：
--
作者：
Schmalenbach I;March TJ;Bringezu T;Waugh R;Pillen K
遗传特征良好的定位群体是快速、精确定位数量性状基因座(QTL)和随后识别潜在基因的关键工具。在本研究中，一组73个导入系(S42IL)起源于春大麦品种Scarlett(Hordeum uggare SSP.)和野生大麦ISR42-8(H.v.SSP.Sphtaneum)用Illumina 1536-SNP阵列进行高分辨率基因分型。该阵列能够精确定位野生大麦在精英大麦背景中的导入。基于636个信息性SNPs，S42IL集合代表了野生大麦基因组的87.3%，其中每个品系平均包含3.3%的供体基因组。此外，为了便于QTL的精细定位和克隆，还建立了70个S42IL的分离高分辨率定位群体(S42IL-HR)。作为案例研究，我们利用已开发的遗传资源，快速鉴定并精细定位了染色体1H上控制籽粒可脱粒性的新基因座位Thresh-1。在这里，隐性野生大麦等位基因赋予了一个难以脱粒的表型，这表明Thresh-1在大麦驯化过程中发挥了重要作用。利用S42IL-HR群体，在4.3 cM的区间内对Thresh-1进行了精细定位，预测该区间包含与植物细胞壁组成调控相关的候选基因。这套野生大麦导入系和衍生的高分辨率群体是加快QTL定位和进一步解剖的理想工具，最终为分离QTL效应的基因扫清了道路。
Genetically well-characterized mapping populations are a key tool for rapid and precise localization of quantitative trait loci (QTL) and subsequent identification of the underlying genes. In this study, a set of 73 introgression lines (S42ILs) originating from a cross between the spring barley cultivar Scarlett (Hordeum vulgare ssp. vulgare) and the wild barley accession ISR42-8 (H. v. ssp. spontaneum) was subjected to high-resolution genotyping with an Illumina 1536-SNP array. The array enabled a precise localization of the wild barley introgressions in the elite barley background. Based on 636 informative SNPs, the S42IL set represents 87.3% of the wild barley genome, where each line contains on average 3.3% of the donor genome. Furthermore, segregating high-resolution mapping populations (S42IL-HRs) were developed for 70 S42ILs in order to facilitate QTL fine-mapping and cloning. As a case study, we used the developed genetic resources to rapidly identify and fine-map the novel locus thresh-1 on chromosome 1H that controls grain threshability. Here, the recessive wild barley allele confers a difficult to thresh phenotype, suggesting that thresh-1 played an important role during barley domestication. Using a S42IL-HR population, thresh-1 was fine-mapped within a 4.3cM interval that was predicted to contain candidate genes involved in regulation of plant cell wall composition. The set of wild barley introgression lines and derived high-resolution populations are ideal tools to speed up the process of mapping and further dissecting QTL, which ultimately clears the way for isolating the genes behind QTL effects.
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