Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans
Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans
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DOI：
10.1093/rheumatology/ken114
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发表时间：
2008-08-01
期刊：
影响因子：
5.5
通讯作者：
Sekiya, I.
中科院分区：
文献类型：
--
作者：
Morito, T.;Muneta, T.;Sekiya, I.
Objective. SF中存在间充质干细胞（MSC）以前有报道。然而，来源于SF的MSC的行为和性质尚未完全阐明。从重建手术前后的19个前交叉韧带损伤的膝关节和3名健康志愿者中获得人类SF。将SF铺板、培养并检查集落形成数、体外分化、表面表位和基因谱。将兔滑膜间充质干细胞注射到兔前交叉韧带部分缺损模型的膝关节内，并对注射的细胞进行追踪。来自IA韧带损伤患者的SF-MSCs的数量是来自健康志愿者的100倍。菌落总数与伤后时间呈正相关。手术前后，SF来源的细胞在表面表位和分化潜力方面没有观察到显着差异。基因图谱聚类分析显示SF-MSCs与滑膜间充质干细胞的相似性高于骨髓间充质干细胞。在兔实验中，注射到IA韧带部分缺损的膝关节中的MSCs在24 h时在缺损区比在韧带完整区更多。我们证明，SF-MSCs，滑膜间充质干细胞类似，IA韧带损伤和手术后，数量增加，而没有显着改变的性质。
Objective. The existence of mesenchymal stem cells (MSCs) in SF was previously reported. However, the behaviour and properties of MSCs derived from SF have not been fully elucidated.Methods. Human SFs were obtained from 19 knee joints with anterior cruciate ligament injury around the time of reconstruction surgery, and from three healthy volunteers. SF was plated, cultured and examined for colony-forming number, in vitro differentiation, surface epitopes and gene profiles. Also, rabbit synovium-MSCs were injected into the knee joint in a rabbit partial anterior cruciate ligament defect model, and the injected cells were traced.Results. SF-MSCs from IA ligament injury patients were 100 times more in number than those from healthy volunteers. Total colony number was positively correlated with post-injury period. No significant differences were observed among the cells derived from SF around the time of the surgery in relation to surface epitopes and differentiation potentials. Cluster analysis of gene profiles demonstrated that SF-MSCs were more similar to synovium MSCs than bone marrow MSCs. In rabbit experiments, the MSCs injected into the knee in which IA ligament was partially defective were observed more on the defected area than on the intact area of the ligament at 24 h.Conclusion. We demonstrated that SF-MSCs, similar to synovium MSCs, increased in number after IA ligament injury and surgery without marked alteration of the properties.