Transcription Analysis of Genes Encoding the Wheat Root Transporter NRT1 and NRT2 Families During Nitrogen Starvation
Transcription Analysis of Genes Encoding the Wheat Root Transporter NRT1 and NRT2 Families During Nitrogen Starvation
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DOI：
10.1007/s00344-014-9435-z
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发表时间：
2014-12-01
影响因子：
4.8
通讯作者：
Kang, Guozhang
中科院分区：
文献类型：
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作者：
Guo, Tiancai;Xuan, Hongmei;Kang, Guozhang
本研究中，氮饥饿8 d显著抑制了小麦幼苗的生长，表现为株高、地上部鲜重和地上部干重下降，但对根系生长有促进作用。氮饥饿显著降低了小麦幼苗叶片和根系中硝酸盐和蛋白质含量，显著提高了氧化胁迫标志物丙二醛含量。摘要鉴定了面包小麦（Triticum aestivum L.） NRT1和NRT2家族的编码基因，并利用实时定量聚合酶链反应在缺氮小麦幼苗根系中测定了它们的转录水平。N饥饿在第2天和第4天显著提高了TaNRT1.1的转录水平；2、4、6天TaNRT1.3；2天TaNRT1.4；TaNRT1.7和TaNRT1.8在2天；TaNRT2.1和TaNRT2.2在2天；和TaNRT2.3在第2天和第4天。然而，TaNRT1.5和TaNRT2.4基因在N饥饿后的所有采样时间点都被显著抑制，而TaNRT1.2和TaNRT2.5基因则被显著诱导。基于这些表达谱，本文讨论了这些转运体在小麦幼苗缺氮过程中的功能。
In the present study, nitrogen (N) starvation for 8 days significantly inhibited the growth of wheat seedlings as manifested by decreased plant height, shoot fresh weight, and shoot dry weight, although it stimulated root growth. The nitrate and protein contents were markedly reduced and the oxidative stress marker, malondialdehyde content, was markedly increased in the leaves and roots of wheat seedlings during N starvation. The genes encoding the NRT1 and NRT2 families in bread wheat (Triticum aestivum L.) were identified, and their transcription levels were measured using quantitative real-time polymerase chain reaction in the roots of N-starved wheat seedlings. N starvation significantly enhanced the transcription levels of TaNRT1.1 at 2 and 4 days; TaNRT1.3 at 2, 4, and 6 days; TaNRT1.4 at 2 days; TaNRT1.7 and TaNRT1.8 at 2 days; TaNRT2.1 and TaNRT2.2 at 2 days; and TaNRT2.3 at 2 and 4 days. However, the TaNRT1.5 and TaNRT2.4 genes were greatly inhibited at all sampling time points after N starvation, whereas the TaNRT1.2 and TaNRT2.5 genes were dramatically induced. The functions of these transporters in N starvation of wheat seedlings based on these expression profiles are herein discussed.