Biosynthetic study of amphidinolide B.
Biosynthetic study of amphidinolide B.
复制标题
氨苯吡啶内酯B的生物合成研究.
DOI：
10.1248/cpb.49.1366
复制
发表时间：
2001
影响因子：
1.7
通讯作者：
J. Kobayashi
中科院分区：
文献类型：
--
作者：
M. Tsuda;T. Kubota;Y. Sakuma;J. Kobayashi
通过对甲藻前沟藻（Amphidinium sp.）培养物进行[1-（13）C]、[2-（13）C]和[1，2-（13）C2]乙酸钠的投喂实验，获得了富含13 C的样品，根据13 C-NMR数据，研究了前沟藻内酯B（1）的生物合成来源。来自乙酸酯的C-2的分离的C1单元，来自乙酸酯的C-2的六个支链C1单元，和仅衍生自乙酸酯的C-2的“m-m”和“m-m-m”单元。amphidinalcine B（1）的标记模式与amphidinalcine H（2）的标记模式不同，amphidinalcine H（2）是一种与1密切相关的26元大环内酯。
The biosynthetic origins of amphidinoide B (1) were investigated on the basis of 13C-NMR data of 13C-enriched samples obtained by feeding experiments with [1-(13)C], [2-(13)C], and [1,2-(13)C2] sodium acetates in cultures of a dinoflagellate Amphidinium sp. These incorporation patterns suggested that 1 was generated from three successive polyketide chains, an isolated C1 unit from C-2 of acetates, six branched C1 units from C-2 of acetates, and an "m-m" and an "m-m-m" unit derived only from C-2 of acetates. The labeling patterns of amphidinolide B (1) were different from those of amphidinolide H (2), a 26-membered macrolide closely related to 1.