ERECTA, an LRR receptor-like kinase protein controlling development pleiotropically affects resistance to bacterial wilt
ERECTA, an LRR receptor-like kinase protein controlling development pleiotropically affects resistance to bacterial wilt
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DOI：
10.1046/j.1365-313x.2003.01877.x
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发表时间：
2003-11-01
期刊：
影响因子：
7.2
通讯作者：
Marco, Y
中科院分区：
文献类型：
--
作者：
Godiard, L;Sauviac, L;Marco, Y
青枯病是世界范围内最具破坏性的植物细菌性病害之一，由青枯病（Ralstonia solanacearum）引起，影响许多重要的作物品种。我们表明，从欧洲茄类作物中分离出的几个菌株在拟南芥的不同品种中具有致病性。其中一个菌株14.25在拟南拟南菌株中引起萎蔫症状，而在哥伦比亚菌株（Col-0）中无明显症状。在易感菌中，疾病的发展和细菌的繁殖取决于功能性超敏反应和致病性（hrp）基因，这是细菌致病性的关键因素。对Ler x col0重组自交系的遗传分析表明，抗性至少由3个位点控制：第2染色体上的QRS1 （Quantitative resistance to R. solanacearum）和QRS2位点，以及第5染色体上的QRS3位点。这些基因座解释了大约90%的col0菌株携带的抗性。ERECTA基因编码一种富含亮氨酸的重复受体样激酶（LRR-RLK）并影响空气器官的发育，在我们的人群中是二态的，位于QRS1附近。经野生型ERECTA基因转化后的易感Ler植株，通过减少枯萎症状和抑制细菌生长，表现出对茄枯病菌的抗性增强，这表明抗性和发育途径之间存在意想不到的交叉作用。
Bacterial wilt, one of the most devastating bacterial diseases of plants worldwide, is caused by Ralstonia solanacearum and affects many important crop species. We show that several strains isolated from solanaceous crops in Europe are pathogenic in different accessions of Arabidopsis thaliana. One of these strains, 14.25, causes wilting symptoms in A. thaliana accession Landsberg erecta (Ler) and no apparent symptoms in accession Columbia (Col-0). Disease development and bacterial multiplication in the susceptible Ler accession depend on functional hypersensitive response and pathogenicity (hrp) genes, key elements for bacterial pathogenicity. Genetic analysis using Ler x Col-0 recombinant inbred lines showed that resistance is governed by at least three loci: QRS1 (Quantitative Resistance to R. solanacearum) and QRS2 on chromosome 2, and QRS3 on chromosome 5. These loci explain about 90% of the resistance carried by the Col-0 accession. The ERECTA gene, which encodes a leucine-rich repeat receptor-like kinase (LRR-RLK) and affects development of aerial organs, is dimorphic in our population and lies close to QRS1. Susceptible Ler plants transformed with a wild-type ERECTA gene, and the LER line showed increased disease resistance to R. solanacearum as indicated by reduced wilt symptoms and impaired bacterial growth, suggesting unexpected cross-talk between resistance and developmental pathways.