Heat shock transcription factor, HSF, is activated by ultraviolet irradiation.
Heat shock transcription factor, HSF, is activated by ultraviolet irradiation.
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DOI：
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发表时间：
1996-12
影响因子：
3.3
通讯作者：
K. Ohnishi;Hideki Matsumoto;A. Takahashi;X. Wang;Takeo Ohnishi
中科院分区：
文献类型：
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作者：
K. Ohnishi;Hideki Matsumoto;A. Takahashi;X. Wang;Takeo Ohnishi
我们以前证明，人胶质母细胞瘤细胞系积累热休克蛋白（HSP）72，不仅在热休克后，但也后，γ射线或紫外线照射。在本研究中，我们调查是否热休克转录因子（HSF）的热休克元件（HSE）的hsp 72基因启动子的结合活性增加后，紫外线照射人胶质母细胞瘤A-172细胞。凝胶迁移率变化分析表明，激活的HSF水平显着增加后，紫外线照射。此外，紫外线照射核提取物在体外没有激活HSF，而在体外热休克处理。这些结果表明，HSF激活可以诱导UV照射在正常的生理温度和热休克蛋白72积累的结果从一个增加的激活HSF水平，即转录上调热休克蛋白72。此外，负责UV诱导的HSF活化的机制可能不同于在热处理细胞中操作的过程。
We demonstrated previously that human glioblastoma cell lines accumulated heat shock protein (hsp)72, not only after heat shock, but also after, gamma-ray or UV irradiation. In the present study, we investigated whether the binding activity of heat shock transcription factor (HSF) to the heat shock element (HSE) of the hsp72 gene promoter increased after UV irradiation of human glioblastoma A-172 cells. A gel mobility-shift assay showed that the activated HSF level increased markedly after UV irradiation. Furthermore, UV irradiation of nuclear extracts in vitro did not activate HSF, whereas in vitro heat shock treatment did. These results suggest that HSF activation can be induced by UV irradiation at normal physiological temperature and hsp72 accumulation results from an increased activated HSF level, i.e. a transcriptional up-regulation of hsp72. In addition, the mechanism responsible for UV-induced HSF activation may differ from the process that operates in heat-treated cells.