MAPPING T-CELL RECEPTOR PEPTIDE CONTACTS BY VARIANT PEPTIDE IMMUNIZATION OF SINGLE-CHAIN TRANSGENICS
MAPPING T-CELL RECEPTOR PEPTIDE CONTACTS BY VARIANT PEPTIDE IMMUNIZATION OF SINGLE-CHAIN TRANSGENICS
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DOI：
10.1038/355224a0
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发表时间：
1992-01-16
期刊：
影响因子：
64.8
通讯作者：
DAVIS, MM
中科院分区：
文献类型：
--
作者：
JORGENSEN, JL;ESSER, U;DAVIS, MM
为了测试T细胞识别的模型，已经用变体肽免疫了T细胞受体α或β链的转基因小鼠，所述变体肽迫使所产生的T细胞应答发生变化。特别地，肽上的电荷取代通常引起T细胞受体V（α）或V（β）链的连接（CDR 3）序列中的相互电荷，表明直接的T细胞受体-肽接触，并允许衍生T细胞受体-MHC相互作用的拓扑结构。在肽上的一个位置，变体将均匀的V（β）反应转化为非常不均匀的反应。
To test models of T-cell recognition, mice transgenic for T-cell receptor-alpha or beta-chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V(alpha) or V(beta) chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V(beta) response into a very heterogeneous one.