THERMO-SENSITIVE SPIKELET DEFECTS 1 acclimatizes rice spikelet initiation and development to high temperature
THERMO-SENSITIVE SPIKELET DEFECTS 1 acclimatizes rice spikelet initiation and development to high temperature
复制标题
热敏小穗缺陷 1 使水稻小穗的萌生和发育适应高温
DOI：
10.1093/plphys/kiac576
复制
发表时间：
2023
期刊：
影响因子：
7.4
通讯作者：
Yuanlin Duan
中科院分区：
文献类型：
--
作者：
Zhengzheng Cai;Gang Wang;Jieqiong Li;Lan Kong;Weiqi Tang;Xuequn Chen;Xiaojie Qu;Chenchen Lin;Yulin Peng;Yang Liu;Zhanlin Deng;Yanfang Ye;Weiren Wu;Yuanlin Duan
摘要：作物生殖发育易受热胁迫影响，然而生殖期尤其是早期耐热性的遗传调控机制仍知之甚少。我们分离出一个禾本科特有的远红光下胚轴伸长3（FAR - RED ELONGATED HYPOCOTYLS3，FHY3）/远红光反应受损1（FAR - RED IMPAIRED RESPONSE1，FAR1）家族转录因子——热敏小穗缺陷1（Thermo - sensitive Spikelet Defects 1，TSD1），它源自水稻（Oryza sativa）转座酶。TSD1在小穗中高表达，受热诱导，且能特异性增强小穗形态发生的耐热性。敲除TSD1不影响营养生长，但显著延缓小穗的起始与发育，并根据温度不同导致不同程度的小穗退化。大多数tsd1小穗在低温下正常，但随温度升高逐渐退化，高温时全部消失，导致枝梗裸露。TSD1直接促进YABBY1和YABBY3的转录，且能与YABBY1以及三个TOB蛋白（YABBY5、YABBY4和YABBY3）发生物理相互作用。这些YABBY蛋白既能形成同源二聚体，也能形成异源二聚体，与TSD1类似，在小穗形态发生中发挥重要作用。值得注意的是，敲除突变体yab5 - ko和双突变体tsd1 yab5 - ko在小穗外观和对温度的响应上与tsd1相似，表明这些基因可能通过TSD1 - YABBY协同模块参与小穗发育。这些发现揭示了FHY3/FAR1家族基因的独特功能以及独特的TSD1 - YABBY复合体，使水稻小穗发育能够适应高温，为深入理解植物生殖发育中耐热性增强的调控途径提供了思路。
Abstract. Crop reproductive development is vulnerable to heat stress, and the genetic modulation of thermo-tolerance during the reproductive phase, especially the early stage, remains poorly understood. We isolated a Poaceae-specific FAR-RED ELONGATED HYPOCOTYLS3 (FHY3)/FAR-RED IMPAIRED RESPONSE1 (FAR1)family transcription factor, Thermo-sensitive Spikelet Defects 1 (TSD1), derived from transposase in rice (Oryza sativa) TSD1 was highly expressed in spikelets, induced by heat, and specifically enhanced the thermotolerance of spikelet morphogenesis. Disrupting TSD1 did not affect vegetative growth but markedly retarded spikelet initiation and development, as well as caused varying degrees of spikelet degeneration, depending on the temperature. Most tsd1 spikelets were normal at low temperature but gradually degenerated as temperature increased, and all disappeared at high temperature, leading to naked branches. TSD1 directly promoted the transcription of YABBY1 and YABBY3 and could physically interact with YABBY1 and three TOB proteins, YABBY5, YABBY4 and YABBY3. These YABBY proteins can form either homodimers or heterodimers and play an important role in spikelet morphogenesis, similar to TSD1. Notably, the knockout mutant yab5-ko and double mutant tsd1 yab5-ko resembled tsd1 in spikelet appearance and response to temperature, indicating that these genes likely participate in spikelet development through the cooperative TSD1-YABBY module. These findings reveal a distinctive function of FHY3/FAR1 family genes and a unique TSD1-YABBY complex to acclimate spikelet development to high temperature in rice, providing insight into the regulating pathway of enhancing thermotolerance in plant reproductive development.