Loss of aquaporin 3 protein expression constitutes an independent prognostic factor for progression-free survival: an immunohistochemical study on stage pT1 urothelial bladder cancer.
Loss of aquaporin 3 protein expression constitutes an independent prognostic factor for progression-free survival: an immunohistochemical study on stage pT1 urothelial bladder cancer.
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DOI：
10.1186/1471-2407-12-459
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发表时间：
2012-10-08
期刊：
影响因子：
3.8
通讯作者：
Denzinger S
中科院分区：
文献类型：
--
作者：
Otto W;Rubenwolf PC;Burger M;Fritsche HM;Rößler W;May M;Hartmann A;Hofstädter F;Wieland WF;Denzinger S
pT 1期尿路上皮膀胱癌（UBC）患者的治疗仍然是一个挑战，由于其不可预测的临床过程。仍然缺乏有助于确定适当个体治疗的可靠分子标记。水通道蛋白（AQP）3蛋白表达的丧失先前已在肌肉侵袭性UBC中显示。本研究的目的是探讨AQP 3蛋白表达对pT 1期UBC预后的预测价值。通过免疫组化研究了87例T1期UBC患者标本中AQP 3蛋白的表达，这些患者在2002年至2009年期间在高容量泌尿外科中心通过经尿道膀胱切除术（TURB）和随后的第二次切除术诊断。患者接受卡介苗（BCG）辅助滴注治疗。AQP 3蛋白表达的丧失被定义为整个肿瘤内完全不存在该蛋白。表达状态与临床病理和随访数据（中位数：31个月）回顾性相关。多变量考克斯回归分析用于评估关于无复发（RFS）、无进展（PFS）和癌症特异性生存（CSS）的AQP 3肿瘤表达的值。通过Kaplan-Meier分析和Log rank检验计算RFS、PFS和CSS。59%的患者显示出AQP 3阳性肿瘤，而41%的肿瘤不表达标记物。AQP 3蛋白表达的缺失与统计学显著较差的PFS相关（20% vs. 72%，p=0.020）。多变量考克斯回归分析证实了这一结果（HR 7.58，CI 1.29 - 44.68; p=0.025）。pT 1 UBC中AQP 3蛋白表达的缺失似乎在疾病进展中起关键作用，并与PFS恶化相关。考虑到其潜在的预后价值，AQP 3蛋白表达的评估可用于帮助对pT 1 UBC患者的行为进行分层。
Treatment of patients with stage pT1 urothelial bladder cancer (UBC) continues to be a challenge due to its unpredictable clinical course. Reliable molecular markers that help to determine appropriate individual treatment are still lacking. Loss of aquaporin (AQP) 3 protein expression has previously been shown in muscle-invasive UBC. The aim of the present study was to investigate the prognostic value of AQP3 protein expression with regard to the prognosis of stage pT1 UBC. AQP 3 protein expression was investigated by immunohistochemistry in specimens of 87 stage T1 UBC patients, who were diagnosed by transurethral resection of the bladder (TURB) and subsequent second resection at a high-volume urological centre between 2002 and 2009. Patients underwent adjuvant instillation therapy with Bacillus Calmette-Guérin (BCG). Loss of AQP3 protein expression was defined as complete absence of the protein within the whole tumour. Expression status was correlated retrospectively with clinicopathological and follow-up data (median: 31 months). Multivariate Cox regression analysis was used to assess the value of AQP3 tumour expression with regard to recurrence-free (RFS), progression-free (PFS) and cancer-specific survival (CSS). RFS, PFS and CSS were calculated by Kaplan-Meier analysis and Log rank test. 59% of patients were shown to exhibit AQP3-positive tumours, whereas 41% of tumours did not express the marker. Loss of AQP3 protein expression was associated with a statistically significantly worse PFS (20% vs. 72%, p=0.020). This finding was confirmed by multivariate Cox regression analysis (HR 7.58, CI 1.29 – 44.68; p=0.025). Loss of AQP3 protein expression in pT1 UBC appears to play a key role in disease progression and is associated with worse PFS. Considering its potential prognostic value, assessment of AQP3 protein expression could be used to help stratify the behavior of patients with pT1 UBC.
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影响因子：
23.4
作者：
Rubenwolf, Peter C.;Georgopoulos, Nikolaos T.;Southgate, Jennifer
通讯作者：
Southgate, Jennifer
影响因子：
4.5
作者：
Otto, Wolfgang;Denzinger, Stefan;Bertz, Simone
通讯作者：
Bertz, Simone
影响因子：
3.6
作者：
Huang, Yiming;Zhu, Zhengcai;Wu, Wenxi
通讯作者：
Wu, Wenxi
DOI：
10.1083/jcb.120.2.371
发表时间：
1993-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Nielsen S;Smith BL;Christensen EI;Knepper MA;Agre P
通讯作者：
Agre P
影响因子：
3.5
作者：
Lopez-Beltran, A;Luque, RJ;Montironi, R
通讯作者：
Montironi, R