DNA RESTRICTION ANALYSIS OF ADENOVIRUS PROTOTYPE-1 TO PROTOTYPE-41
DNA RESTRICTION ANALYSIS OF ADENOVIRUS PROTOTYPE-1 TO PROTOTYPE-41
复制标题
DOI：
10.1007/bf01314287
复制
发表时间：
1986-01-01
影响因子：
2.7
通讯作者：
WIGAND, R
中科院分区：
文献类型：
--
作者：
ADRIAN, T;WADELL, G;WIGAND, R
所有已知的人腺病毒原型（1至41）的DNA限制性内切模式，根据亚属A到F排序，提出了限制性内切酶BamHI， BgIII, BstEII， HindIII和SmaI。本目录是在诊断实验室通过DNA限制分析进行腺病毒分型和在参考实验室进行菌株鉴定的先决条件。为了确定人类腺病毒在一个亚属内和亚属之间的遗传关系，对同源片段进行了两两分析。C亚属腺病毒具有密切的亲缘关系。B亚属腺病毒表现出两个相关的聚类，每个聚类有四种类型，而D亚属的众多血清型没有表现出任何相应的聚类。A亚属成员和F亚属成员的DNA限制性片段之间几乎没有相互迁移。
DNA restriction patterns of all known human adenovirus prototypes (1 to 41), ordered according to subgenera A to F, are presented for restriction endonucleases BamHI, BgIII, BstEII, HindIII, and SmaI. This catalogue is a prerequisite for typing of adenoviruses by DNA restriction analysis in diagnostic laboratories and for strain identification in reference laboratories. To determine the genetic relationship of human adenoviruses within a subgenus and between subgenera, a pairwise analysis of comigrating fragments was performed. Adenoviruses of subgenus C were closely related. Adenoviruses of subgenus B showed two related clusters of four types each, whereas the numerous serotypes of subgenus D did not show any corresponding clustering. Little comigration was observed between DNA restriction fragments from members of subgenus A or F respectively.