Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.)
Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.)
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DOI：
10.1271/bbb.63.302
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发表时间：
1999-02-01
影响因子：
1.6
通讯作者：
Tanaka, K
中科院分区：
文献类型：
--
作者：
Kaminaka, H;Morita, S;Tanaka, K
我们从水稻（Oryza sativa L.）中分离出了编码 Fe-SOD 的 cDNA。推导的氨基酸序列由具有 255 个氨基酸的多肽组成，其中包括氨基末端残基中的推定转运肽（40 个氨基酸）。该序列与已知的植物 Fe-SOD 相似，但不属于已知的 Fe-SOD 组。对大肠杆菌中表达的部分纯化的重组蛋白进行金属分析和 Son 分析表明，该 cDNA 编码含铁 SOD。然而，这种 SOD 活性并未被过氧化氢处理所抑制，而过氧化氢预计会抑制已知的 Fe-SOD 活性。在所有检查的营养组织中均检测到水稻 Fe-SOD mRNA，在愈伤组织中尤其丰富，并且通过光诱导强烈增加。这些结果表明该 cDNA 编码水稻 Fe-SOD，这明显不同于已知的植物 Fe-SOD。
We have isolated a cDNA encoding Fe-SOD from rice (Oryza sativa L.). The deduced amino acid sequence consists of a polypeptide with 255 amino acids, including a putative transit peptide (40 a.a.) in amino-terminal residues. This sequence is similar to the known plant Fe-SODs but not classified in the group of known Fe-SODs. The metal analysis and Son assays of the partial purified recombinant protein expressed in E. coli showed that this cDNA encodes an iron-containing SOD. However this SOD activity was not inhibited by the treatment with hydrogen peroxide, which was expected to inhibit known Fe-SOD activity. mRNA of rice Fe-SOD was detected in all vegetative tissues examined, being especially abundant in calli, and strongly increased by light induction. These results suggested that this cDNA encodes rice Fe-SOD, which is apparently distinct from known plant Fe-SODs.