Evidence for DNA damage in patients with coronary artery disease
Evidence for DNA damage in patients with coronary artery disease
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DOI：
10.1016/s1383-5718(01)00162-0
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发表时间：
2001-06-27
影响因子：
1.9
通讯作者：
Andreassi, MG
中科院分区：
文献类型：
--
作者：
Botto, N;Rizza, A;Andreassi, MG
根据动脉粥样硬化的“单克隆假说”，一些研究表明，癌症和动脉粥样硬化可能具有一些共同的基本生物学机制。因此，突变率的增加可能与动脉粥样硬化斑块的发病机制有关。该研究的目的是通过微核（MN）试验验证冠状动脉疾病患者外周血淋巴细胞中染色体损伤的存在，微核（MN）试验是一种可靠的遗传和癌症风险评估生物标志物。受试者包括53例记录在案的冠状动脉缺血性心脏病患者（I组）；10例无冠状动脉粥样硬化病变的瓣膜性心脏病患者（II组）和16例年龄和性别匹配的健康受试者（III组）作为对照。对每个受试者进行两次单独培养，并对1000个双核细胞进行评分，以评估MN频率。MN频率的平均值（+/- s.e.m.）分别为11.9 +/- 1.7、5.9 +/- 1.2和3.6 +/- 0.7。I组的MN频率显著高于m组（P = 0.02）。在第一组中，MN频率随着受影响血管数量的增加而增加（一血管、二血管和三血管疾病分别为6.3 +/- 0.7、13.9 +/- 1.6、14.9 +/- 5.3）。Scheffe’s检验显示，两支血管病变的MN频率明显高于单支血管病变（P = 0.0077）。此外，通过Duke评分系统计算，MN水平与疾病严重程度（R = 0.28, P = 0.032）以及收缩压（R = 0.34, P = 0.009）呈正相关。这些结果表明，人类冠状动脉疾病是一种以DNA损伤增加为特征的疾病，与动脉粥样硬化疾病的严重程度呈正相关。(C) 2001 Elsevier Science B.V.版权所有
According to the "monoclonal hypothesis" of atherosclerosis, several studies suggest that cancer and atherosclerosis may have several fundamental biological mechanisms in common. Therefore, an increase in the mutation rate may be involved in the pathogenesis of atherosclerotic plaques.The aim of the study was to verify the presence of chromosomal damage in peripheral blood lymphocytes in patients with coronary artery disease by using micronucleus (MN) test, a reliable biomarker in genetic and cancer risk assessment.Subjects included 53 patients with documented coronary ischemic heart disease (group I); 10 patients with valvular heart disease in absence of atherosclerotic lesions of the coronary arteries (group II) and 16 healthy subjects, age- and sex-matched (group III) were studied as controls. For each subject, two separate cultures were performed and 1000 binucleated cells were scored for the evaluation of MN frequency.The mean (+/-S.E.M.) of MN frequency were 11.9 +/- 1.7, 5.9 +/- 1.2 and 3.6 +/- 0.7 in groups I, II and III, respectively. The MN frequency of group I was significantly higher than that of group m (P = 0.02). In group I, MN frequency increased with the number of affected Vessels (6.3 +/- 0.7, 13.9 +/- 1.6, 14.9 +/- 5.3 for one-, two-, and three-vessel disease, respectively). Scheffe's test showed that MN frequency was significantly higher in two-vessel compared with one-vessel disease (P = 0.0077). Moreover, a positive relationship was found between MN levels and the severity of the disease, calculated by the Duke scoring system (R = 0.28, P = 0.032), as well as the systolic blood pressure (R = 0.34, P = 0.009).These results suggest that coronary artery disease in humans is a condition characterized by an increase of DNA damage, positively correlated with the severity of the atherosclerotic disease. (C) 2001 Elsevier Science B.V. AII rights reserved.