Tau protein binds single-stranded DNA sequence specifically - the proof obtained in vitro with non-equilibrium capillary electrophoresis of equilibrium mixtures
Tau protein binds single-stranded DNA sequence specifically - the proof obtained in vitro with non-equilibrium capillary electrophoresis of equilibrium mixtures
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DOI：
10.1016/j.febslet.2005.01.032
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发表时间：
2005-02-28
期刊：
影响因子：
3.5
通讯作者：
Krylov, SN
中科院分区：
文献类型：
--
作者：
Krylova, SM;Musheev, M;Krylov, SN
Tau蛋白是一种微管相关蛋白，在神经元的生理和病理过程中起着重要作用。最近报道Tau结合双链DNA（dsDNA）但不结合单链DNA（ssDNA）[Cell.摩尔Life Sci. 2003，60，413-421]。在这里，我们证明tau不仅结合dsDNA，而且结合ssDNA。这一发现是通过使用两种动力学毛细管电泳方法：（i）非平衡毛细管电泳的平衡混合物（NECEEM）;（ii）亲和介导的NECEEM。使用这种新方法，我们首次观察到tau可以通过以序列特异性方式结合其中一条链来诱导dsDNA链的解离。此外，我们确定了所有研究的tau-DNA复合物的平衡解离常数。(C)2005年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Tau is a microtubule-associated protein, which plays an important role in physiology and pathology of neurons. Tau has been recently reported to bind double-stranded DNA (dsDNA) but not to bind single-stranded DNA (ssDNA) [Cell. Mol. Life Sci. 2003, 60, 413-421]. Here, we prove that tau binds not only dsDNA but also ssDNA. This finding was facilitated by using two kinetic capillary electrophoresis methods: (i) non-equilibrium capillary electrophoresis of equilibrium mixtures (NECEEM); (ii) affinity-mediated NECEEM. Using the new approach, we observed, for the first time, that tau could induce dissociation of strands in dsDNA by binding one of them in a sequence-specific fashion. Moreover, we determined the equilibrium dissociation constants for all tau-DNA complexes studied. (C) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.