Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile
Sensitivity and Kinetics of an NS1-Based Zika Virus Enzyme-Linked Immunosorbent Assay in Zika Virus-Infected Travelers from Israel, the Czech Republic, Italy, Belgium, Germany, and Chile
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DOI：
10.1128/jcm.00346-17
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发表时间：
2017-06-01
影响因子：
9.4
通讯作者：
Schwartzh, Eli
中科院分区：
文献类型：
--
作者：
Lustig, Yaniv;Zelena, Hana;Schwartzh, Eli
由于寨卡病毒与其他黄病毒抗体的高交叉反应性，寨卡病毒的血清学诊断具有挑战性。最近，一种基于寨卡病毒NS 1的酶联免疫吸附试验（ELISA）被开发出来，并被证明对寨卡病毒抗体检测具有高度特异性;然而，仅对少数确诊的寨卡病毒感染患者进行了灵敏度评估。在这项研究中，我们使用基于Zika NS 1的ELISA对来自以色列、捷克共和国、意大利、比利时、德国和智利的63名感染寨卡病毒（通过PCR或中和试验证明）的回国旅行者的105份样本进行了寨卡IgM和IgG抗体的敏感性和动力学测量。寨卡病毒IgM在症状发作后2至42天检测到，第一个月的总体敏感性为79%，在2个月的PSO中为68%，而IgG抗体在5天至3年的PSO中检测到，敏感性为79%。有趣的是，在以色列和欧洲/智利寨卡病毒感染的旅行者之间观察到IgM敏感性和IgM检测时间的显著差异，增加了寨卡病毒感染诊断的复杂性，并表明应补充其他诊断方法以减少假阴性结果。
Serological diagnosis of Zika virus is challenging due to high cross-reactivity of Zika virus with other flavivirus antibodies. Recently, a Zika NS1-based enzyme-linked immunosorbent assay (ELISA) was developed and shown to be highly specific for Zika antibody detection; however, sensitivity was evaluated for only a small number of confirmed Zika-infected patients. In this study, we measured the sensitivity and kinetics of Zika IgM and IgG antibodies using the Zika NS1-based ELISA in 105 samples from 63 returning travelers infected with Zika virus (proven by PCR or neutralization assay) from Israel, Czech Republic, Italy, Belgium, Germany, and Chile. Zika virus IgM was detected from 2 to 42 days post-symptom onset (PSO) with an overall sensitivity of 79% in the first month and 68% until 2 months PSO, while IgG antibodies were detected from 5 days to 3 years PSO with 79% sensitivity. Interestingly, significant differences in IgM sensitivity and IgM detection period were observed between Israeli and European/Chilean Zika-infected travelers, adding to the complexity of Zika infection diagnosis and suggesting that other diagnostic methods should be complemented to reduce false-negative results.